miR-885-3p抑制胃癌细胞的增殖和迁移

miR-885-3p Inhibits Proliferation and Migration of Gastric Cancer Cells

目的探讨微小RNA miRNA-885-3p对胃癌细胞增殖和迁移的影响。方法采用qRT-PCR检测miRNA-885-3p在4种胃癌细胞株(SGC-7901、MGC-803、BGC-823、AGS)以及人胃正常黏膜GES-1细胞中的表达。将mimic(miRNA-885-3p模拟物)或miRNA-NC转染SGC-7901细胞后,分为3组:miR-mimic组(mimic转染)、miR-NC组(miRNA-NC转染)、空白对照组(未经转染),采用CCK-8检测各组SGC-7901细胞增殖情况,采用伤口愈合实验检测各组SGC-7901细胞迁移能力,采用transwell实验检测各组SGC-7901细胞侵袭能力。结果miRNA-885-3p在SGC-7901、MGC-803、BGC-823、AGS胃癌细胞株中的表达显著低于在人胃正常黏膜GES-1细胞中的表达(P<0.05),在miR-NC组、空白对照组SGC-7901细胞中的表达显著低于在miR-mimic组SGC-7901细胞中的表达(P<0.05)。miR-mimic组SGC-7901细胞活力、迁移距离及侵袭细胞数均显著低于空白对照组和miR-NC组(均P<0.05)。结论miRNA-885-3p在胃癌细胞中低表达,其抑制胃癌细胞增殖和迁移,具有潜在的应用价值。

Objective To investigate the effect of miRNA-885-3p on the proliferation and migration of gastric cancer cells.Methods The expression of miRNA-885-3p was detected by qRT-PCR in four gastric cancer cell lines(SGC-7901,MGC-803,BGC-823,AGS)and normal gastric mucosa GES-1 cells.SGC-7901 cells were divided into three groups:miRNA-885-3p mimic transfection group,miR-NC transfection group and blank control group.The proliferation,migration and invasion of SGC-7901 cells were determined by CCK-8 assay,wound healing assay and transwell assay,respectively.Results The expression of miRNA-885-3p in SGC-7901,MGC-803,BGC-823 and AGS cells was lower than that in GES-1 cells,and that in miR-NC transfection group and blank control group was lower than that in miRNA-885-3p mimic transfection group(P<0.05).Furthermore,compared with miR-NC transfection group and blank control group,the viability,migration distance and number of invaded cells decreased in miRNA-885-3p mimic transfection group(P<0.05).Conclusion miRNA-885-3p is lowly expressed in gastric cancer cells,and can inhibit the proliferation and migration of gastric cancer cells.Therefore,it is of potential value in the treatment of gastric cancer.