草地贪夜蛾细胞株Sf 9生测杀虫剂毒力的方法探索

Evaluating insecticide bioassay methods using the Spodoptera frugiperda cell line Sf 9

【目的】为适应杀虫剂新产品研发和害虫抗药性监测对快速生物测定的需要,本文探索细胞生测与试虫生测的关联情况。【方法】分别测定草地贪夜蛾Spodoptera frugiperda卵巢细胞株(Sf 9细胞)和小菜蛾Plutellaxyllostella敏感品系对不同类型的杀虫剂的毒力,并在此基础上进行了细胞活力抑制率与活体昆虫致死率的关联性分析。【结果】3种不同机理杀虫剂毒力在2种生测体系中均表现出阿维菌素>吡虫啉>高效氯氰菊酯的一致规律;同一类型(有机磷类)5种杀虫剂毒力在2种生测体系中均表现出马拉硫磷>敌敌畏>辛硫磷>丙硫磷>敌百虫的一致规律;同一种类(5%阿维菌素)3种剂型杀虫剂毒力在2种生测体系中均表现出了微乳剂>乳油>水乳剂的一致规律。细胞生测和试虫生测之间存在高度相关性,据此建立不同杀虫剂细胞8 h生测和试虫48 h生测剂量之间的线性回归方程。【结论】建立了不同杀虫剂处理Sf9细胞8h与处理小菜蛾幼虫48h的线性相关模型,可根据细胞生物测定结果反映试虫生物测定的结果,为今后新型农药的筛选、毒力测定等提供便利的条件。

[Objectives]To meet the need for rapid bioassay methods for pesticide development and pest resistance monitoring,we compared the results obtained from bioassays using insect cells vs whole insects.[Methods]The virulence of different pesticides to the Spodoptera frugiperda ovary cell line(Sf 9)and sensitive strains of Plutella xylostella was examined,and the correlation between the inhibition of the cell proliferation rate and insect mortality compared.[Results]Both bioassay systems ranked the virulence of the three different types of insecticides as avermectin>imidacloprid>betacypermethrin,and the virulence of the five insecticides in the same category(organophosphorus)as>dichlorvos>phoxim>propionate>trichlorfon.Both bioassay systems also ranked the virulence of three formulations of the same insecticide(5%avermectin)as micro-emulsionn>emulsifiable concentrate>emulsion.A linear regression model of the relationship between an 8 h cell bioassay and 48 h insect bioassay was constructed.[Conclusion]The results of an 8 h cell bioassay were consistent with those of a 48 h insect bioassay.Cell bioassays provide a relatively quick way of testing new pesticides.