掺铜介孔生物活性玻璃的制备及其生物活性

Preparation of copper-doped mesoporous bioglass and its bioactivity

采用溶胶-凝胶法分别以溴化十六烷基三甲铵(CTAB)、正硅酸乙酯(TEOS)、硝酸钙、铜/抗坏血酸复合物作为玻璃前驱体合成掺铜介孔生物玻璃,通过TEM、FTIR及氮气吸附法等测试方法表征该玻璃材料的表面形态、化学结构和理化性质,并测定不同剂量掺铜梯度对介孔生物玻璃粉体的生物活性影响。结果表明:选择不同比例的铜/抗坏血酸复合物可以获得可控的掺铜介孔生物玻璃纳米球,其中样品1Cu-MBG、5Cu-MBG、7Cu-MBG的孔容分别为0.62、0.74 m^(3)/g和0.78 m^(3)/g,孔径分别为6.91、7.28 nm和10.06 nm,随着Cu含量的增加,Cu-MBGs样品的孔容和平均孔径均增加;所有样品颗粒均表现出较大的比表面积、孔容和孔径,且体外生物活性良好。所制备的玻璃材料有望应用于骨组织再生修复和皮肤缺损愈合。

Copper-doped mesoporous bioglass was synthesized with sol-gel method by using cetyltrimethylammonium bromide(CTAB),ethyl orthosilicate(TEOS),calcium nitrate,and copper/ascorbic acid composite as glass precursors.And TEM,FTIR and nitrogen adsorption method were used to characterize the surface morphology,chemical structure,physical and chemical properties of the glass material.The effect of different doses of copper doping gradient on the bioactivity of mesoporous bioglass powder was explored.The results showed that controllable copper-doped mesoporous bioglass nanospheres could be obtained by choosing different proportions of copper/ascorbic acid composites.The pore volume of samples 1Cu-MBG,5 Cu-MBG,7 Cu-MBG was 0.62,0.74 and 0.78 m^(3)/g respectively,and the pore size was 6.91,7.28,10.06 nm respectively.As the copper content increased,the pore volume and average pore size of Cu-MBGs samples increased.All sample particles showed large specific surface area,pore volume and pore size,with good in vitro bioactivity.The glass material prepared is expected to be further applied in bone tissue regeneration,repair and skin defect healing.