实时荧光定量PCR结合培养法在细菌学室间质量评价中的应用

Application of real-time PCR methods and the culture methods in the bacteriology external quality assessment

目的评价培养法和实时荧光PCR法在细菌学质控样本中的检测应用。方法采用培养法和实时荧光PCR法分离鉴定30件质控考核致病菌,探讨快速、准确检测致病菌的理想方案。结果从鉴定结果和检测完成时间方面具体分析了其中5件质控标本,质控标本1号、2号实时荧光PCR优于培养法,质控标本3号、4号培养法弥补了实时荧光PCR的局限性,质控标本5号培养法和实时荧光PCR结合应用,检出目标菌。结论培养法结合实时荧光PCR法能够优势互补,是快速准确检测致病菌的理想模式。

Objective To evaluate the application of culture method and real-time PCR method in the detection of multi-blind external quality assessment.Methods Thirty samples of pathogenic bacteria were isolated and identified by culture method and real-time fluorescence PCR method,and the ideal scheme of rapid and accurate detection of pathogenic bacteria was discussed.Results Five specimens were analyzed in detail from the identification results and the completion time of the test.For specimens No.1 and No.2,real-time PCR was better than culture method.The limitations of real-time fluorescent PCR were fulfilled by culture methods in specimen No.3 and No.4.The target bacteria were detected by the combination of quality control specimen No.5 culture method and real-time fluorescence PCR.Conclusion The culture method combined with real-time PCR is an ideal model for rapid and accurate detection of pathogenic bacteria.