2种检测方法对弯曲菌检出率的影响

Effects of 2 kinds of detection methods on the detection rate of Campylobacter

目的比较国标法和双孔滤膜法在生禽肉中弯曲菌的检测率,探索生禽肉中弯曲菌分离鉴定的有效检测方法。方法随机采集冷冻和新鲜禽肉产品共102份,通过国标法和双孔滤膜法2种方法检测,观察2种方法从样品处理、增菌和分离培养过程中的不同,并比较2种方法检测弯曲菌检出率的差异。结果102份样品中国标法检出弯曲菌的检出率为28.43%,其中空肠弯曲菌和结肠弯曲菌的检出率分别为13.725%和14.705%;双孔滤膜法检出弯曲菌的检出率为53.92%,其中空肠弯曲菌和结肠弯曲菌的检出率分别为26.47%和27.45%;双孔滤膜法检出率显著高于国标法,差异有统计学意义(P<0.05)。结论应用双孔滤膜法能显著提高生禽肉食品中弯曲菌的检出率。

Objective To compare the detection rate of Campylobacter in raw poultry meat by the national standard method and the double-pore filter membrane method,and explore an effective detection methods for the isolation and identification of Campylobacter in raw poultry meat.Methods A total of 102 samples of frozen and fresh poultry products were randomly collected and tested by the national standard method and the double-pore filter membrane method.The differences of the 2 methods in the process of sample treatment,bacteria increasing and culture separation were observed,and the differences of the 2 methods in the detection rate of Campylobacter were compared.Results The detection rate of Campylobacter in 102 samples by Chinese standard method was 28.43%,Campylobacter jejuni and Campylobacter coli were 13.725%and 14.705%,respectively.The detection rate of Campylobacter by the double-pore filter membrane method was 53.92%,Campylobacter jejuni and Campylobacter coli were 26.47%and 27.45%,respectively.The detection rate of the double-pore filter membrane method was significantly higher than that of the national standard method,and the difference was statistically significant(P<0.05).Conclusions The application of double-pore filter membrane method can significantly improve the detection rate of Campylobacter in raw poultry food.