基于微卫星标记的斑点叉尾鮰家系鉴定技术及应用

ESTABLISHMENT AND APPLICATION OF PEDIGREE IDENTIFICATION TECHNIQUE FOR CHANNEL CATFISH BASED ON MICROSATELLITE MARKERS

研究旨在建立准确、高效且经济的斑点叉尾鮰(Ictalures punctatus)家系亲缘鉴定体系,以期为斑点叉尾鮰的遗传评估及家系育种提供科学依据。选用10对具有较高多态性SSR标记,建立两个5重PCR反应体系。应用建立的斑点叉尾鮰家系亲缘鉴定体系对来源于13个全同胞家系和8个半同胞家系的333尾个体进行亲权鉴定。结果表明:10个位点平均等位基因数为9.8、平均观测杂合度0.8591、平均期望杂合度0.8092、平均多态信息含量0.7845;3种情况下的累积排除概率分别为:0.99996806、0.99833267和0.99999998;验证群体的鉴定结果与系谱高度一致,真实鉴定率达到99.1%,子代与父母本三者之间配对平均LOD值介于13.30—24.70,且置信度均达到95%。研究选择的微卫星位点等位基因数目较多,多态性较高,可以快速、准确地对斑点叉尾鮰混养群体进行家系鉴定。

To establish an accurate,efficient and economical pedigree identification technique for the channel catfish and provide a scientific basis for the genetic evaluation of the channel catfish and family breeding,two 5-plex PCR reactions were established using 10 pairs of SSR markers with high polymorphism.333 individuals from 13 full-sib families and 8 half-sib families were identified by the channel catfish pedigree identification system.The results showed that the average number of alleles at 10 loci was 9.8,the average observed heterozygosity was 0.9952,the average expected heterozygosity was 0.8092,and the average polymorphism information content was 0.7845.The cumulative exclusion probabilities of these three cases were 0.99996806,0.99833267,and 0.99999998,respectively.The identification of the verified population was highly consistent with the pedigree with a true identification rate of 99.1%.The average LOD value between the offspring and the parents was between 13.30 and 24.70,with a confidence level of 95%.The number of alleles in the microsatellite locus selected in this study is abundant,and the polymorphism is high.Our pedigree identification system can quickly and accurately identify the family from the channel catfish breeding population.