急性高温胁迫对大口黑鲈“优鲈3号”肝脏凋亡相关酶活性和基因表达的影响

Effect of high-temperature stress on apoptosis-related enzyme activities and gene expression in the liver of Micropterus salmoides“youlu No.3”

为了探究急性高温胁迫对大口黑鲈“优鲈3号”凋亡相关酶活性和基因表达的影响,将25℃下暂养的大口黑鲈“优鲈3号”直接放入37℃水环境中,探究急性高温胁迫后幼鱼肝脏组织Caspase3、Caspase9和基因酶活的变化。结果显示:37℃高温胁迫后,肝脏Caspase3和Caspase9酶活均显著升高,且随着胁迫时间的延长呈升高趋势,在48 h达到峰值。基因Caspase3和Caspase9的表达量均呈现先升高再下降的趋势,在6 h的表达量达到峰值,随之下降,并在48 h与对照组无显著性差异。BCL-2的表达量于1 h达到峰值,之后显著下降,48 h时BCL-2的表达量仅为对照组的0.04倍。结果表明,高温胁迫会使大口黑鲈“优鲈3号”幼鱼发生凋亡甚至细胞坏死。

Micropterus salmoides“youlu No.3”were acclimated at 25℃for 3 weeks then treated at 37℃through acute temperature stress.Meanwhile,Caspase3 and Caspase9 enzyme activities and Caspase3,Caspase9 and BCL-2 gene expression in the liver were examined.The results showed that the activities of Caspase3 and Caspase9 in the liver increased significantly,and showed a rising trend with the prolongation of the stress time,reaching the peak at 48h after high-temperature stress at 37℃.The expression levels for both of genes Caspase3 and Caspase9 increased first and then decreased.The expression levels peaked at 6 h,and then decreased at 48 h.There was no significant difference from the control group.The expression level of BCL-2 reached its peak at 1 h,and then significantly decreased to 48 h after the expression level of BCL-2 was only 0.04 times that of the control group.High-temperature stress could cause apoptosis and even cell necrosis in juvenile M.salmoides“Youlu No.3”.Therefore,we should pay close attention to the rapid change of temperature and take appropriate measures to reduce the damage of temperature fluctuation to fish in actual aquaculture production,especially in the face of extreme weather.