3q26 rs12696304基因多态性与中国南方汉族老年人群急性心力衰竭患者一年预后间的关系研究

Correlation between gene polymorphism of chromosome 3q26 rs12696304 and 1-year survival rate after acute heart failure in an elderly Han population in South China

目的:研究3q26 rs12696304基因多态性与急性心力衰竭(急性心衰)患者1年预后间的关系。方法:2012年3月至2016年4月间,收集南京医科大学第一附属医院收治的336例急性心衰患者(≥60岁)。采用TaqMan荧光探针实时定量聚合酶链式反应(polymerase chain reaction,PCR)技术法检测患者外周血DNA中的3q26 rs12696304基因多态性,采用Kaplan-Meier法和log-rank检验,分析不同遗传模型下各基因型急性心衰患者1年的生存率;采用多因素Cox回归分析探讨3q26 rs12696304基因多态性与急性心衰患者1年预后间的相关性。结果:在共显性遗传模型中,携带杂合子CG基因型的急性心衰患者其1年生存率(74.8%)比携带纯合子GG基因型患者(85.4%)低(CG比GG,P=0.027);在显性遗传模型中,携带CG+CC基因型急性心衰患者其生存率(74.9%)较纯合子GG基因型患者生存率(85.4%)明显下降(P=0.023),CC+CG基因型急性心衰患者发生死亡的风险系数是GG基因型患者的2.45倍[风险比(hazard ratio,HR)=2.45,95%CI为1.24~4.84,P=0.010]。结论:检测3q26 rs12696304基因多态性可能有助于评估中国南方汉族老年人群中急性心衰患者1年的预后情况。

Objective:To investigate the correlation between the gene polymorphism of rs12696304 in chromosome 3q26 and 1-year survival rate in the patients with acute heart failure(AHF).Methods:A total of 336 elderly patients of Han population in South China(aged≥60 years)with AHF were enrolled during March 2012 and April 2016.DNA of each patient was extracted from peripheral blood,and the gene polymorphism of rs12696304 was analyzed by real-time fluorescence quantitative PCR with TaqMan probe.One-year survival rate was compared between the patients with different gene typing in multiple genetic models using Kaplan-Meier and log-rank test.The Cox regression analysis was used to assess relation between the gene polymorphism of rs12696304 and 1-year survival rate in the patients with AHF.Results:In the co-dominant genetic model,the 1-year survival rate of the AHF patients carrying heterozygous CG genotypes was lower than that of patients carrying wild homozygous GG genotypes(74.8%vs 85.4%,P=0.027).In the dominant genetic model,1-year survival rate of the AHF patients carrying CG+CC genotype was significantly lower than that of patients carrying the wild homozygous GG genotype(74.9%vs 85.4%,P=0.023).Further Cox regression analysis showed that the risk of 1-year death in the AHF patients carrying CG+CC genotype was 2.45 times of that in the patients with GG genotype(HR=2.45,95%CI:1.22-4.95,P=0.012)after adjustment with the relevant variables in codominant model,and in the dominant model,the risk of 1-year death was also 2.45 times of that in patients with GG genotype(HR=2.45,95%CI:1.24-4.84,P=0.010).Conclusions:The gene polymorphisms of rs12696304 located in chromosome 3q26 region may be associated with the 1-year prognosis in the patients with AHF.