日光温室作业人员血清中基质金属蛋白-3和血管内皮生长因子的水平分析

Analysis on levels of serum MMP-3 and VEGF in greenhouse workers

目的分析日光温室作业人员血清中基质金属蛋白-3(MMP-3)和血管内皮生长因子(VEGF)的水平、分布特征及可能的影响因素。方法选择西北某地区蔬菜种植人员861人作为研究对象,其中温室作业(温室组)566人、非温室作业(非温室组)295人,检查骨关节炎(OA)患病情况。采集空腹静脉血,采用ELISA法测定血清中MMP-3、VEGF浓度,分析温室作业及其他多种因素对血清MMP-3、VEGF浓度的影响。结果温室组OA患病率为29.86%,显著高于非温室组(7.80%)。温室组血清MMP-3浓度高于非温室组且差异有统计学意义(P<0.05),血清VEGF浓度升高但差异无统计学意义(P>0.05)。温室组MMP-3浓度[M(P_(25),P_(75))]为15.32(10.20,23.52)ng/ml,>50岁组MMP-3水平显著高于≤50岁组(P<0.05),吸烟、饮酒者MMP-3浓度明显高于非吸烟、非饮酒者(P<0.05);不同从业时间组间血清MMP-3水平差异有统计学意义(P<0.05),并随工龄增长而升高(r=0.105,P<0.05)。温室组血清VEGF浓度[M(P_(25),P_(75))]为279.24(201.15,418.30)pg/ml,吸烟、饮酒者明显高于非吸烟、非饮酒者(P<0.05)。非温室组血清MMP-3、VEGF浓度[M(P_(25),P_(75))]分别为12.70(9.33,21.35)ng/ml和266.54(168.78,400.57)pg/ml。多重线性回归结果表明,高暴露水平的温室作业、性别和饮酒是影响血清MMP-3水平的因素,中、高暴露水平的温室作业以及体重指数(BMI)对血清VEGF水平有影响。结论日光温室作业人员OA患病率显著升高。温室作业累计暴露水平、饮酒、BMI是影响血清MMP-3和VEGF水平的因素,提示MMP-3和VEGF可能作为日光温室作业人员骨关节损伤的生物标志物。

Objective To analyze the serum levels and distribution characteristics of matrix metalloproteinase-3(MMP-3)and vascular endothelial growth factor(VEGF),and possible influencing factors in the greenhouse workers.Methods 861 vegetable growers in northwest China were selected as the research objects,including 566 greenhouse workers and 295 non-greenhouse workers.The prevalence of osteoarthritis in these workers was examined,and the fasting venous blood was collected for detecting of MMP-3 and VEGF in the serum by ELISA,and then,analyze the effects of greenhouse operation and other factors on serum levels of MMP-3 and VEGF.Results The results showed that the prevalence of osteoarthritis in greenhouse workers was 29.86%,which was significantly higher than that in non-greenhouse workers(7.80%).Compared with the non-greenhouse group,the serum level of VEGF has not shown significant difference between two groups,but the serum level of MMP-3 of greenhouse group was significantly increased(P<0.05).In greenhouse group,the median and interquartile range of the serum MMP-3 level were 15.32 and 10.20,23.52 ng/ml,respectively,over 50 s age group had higher serum level of MMP-3,and the serum MMP-3 levels in smokers and drinkers were also significantly higher than that in non-smokers and non-drinkers(P<0.05),furthermore,the differences of serum MMP-3 levels among different working age groups were statistically significant as well(P<0.05),which showed some increasing trends with the of working age(r=0.105,P<0.05).While the median and interquartile range of serum VEGF were 279.24 and 201.15,418.30 pg/ml,respectively,the serum levels in smokers and drinkers were also significantly higher than that of non-smokers and non-drinkers(P<0.05).In non-greenhouse group,the median and interquartile range of the serum MMP-3 level were 12.70 and 9.33,21.35 ng/ml,VEGF level were 266.54 and 168.78,400.57 pg/ml,respectively.Multiple linear regression analysis showed that higher exposure to greenhouse operation,gender and drinking were the main influencing factors of serum MMP-3 level;additionally,the higher or medium exposure to greenhouse operation and BMI also showed some effects on serum VEGF level.Conclusion The results suggested that the prevalence of osteoarthritis was significantly increased,the cumulative exposure level to greenhouse operation,drinking and IBM were the main influenceing factors of serum MMP-3 and VEGF levels,which suggests that MMP-3 and VEGF may be taken as biomarkers of osteoarticular injury of greenhouse workers.