基于网络药理学的僵蚕主要药效作用研究

Research on the Main Pharmacodynamics of Bombyx Batryticatus Based on Network Pharmacology

目的:通过网络药理学方法探讨僵蚕的主要药效作用机制。方法:利用化学专业数据库及文献收集僵蚕的化学成分,借助中药系统药理学数据库和分析平台(TCMSP)以及Swiss Target Prediction数据库获得僵蚕化合物潜在作用靶点,结合TTD、DisGeNET等生物信息库及僵蚕现代药理作用将靶标映射在相关疾病上。运用Cytoscape构建药材-成分-靶点-疾病网络模型,并应用DAVID数据库基于KEGG通路分析僵蚕作用机制。结果:僵蚕39个化学成分共作用451个靶点,主要富集在糖尿病、哮喘、肿瘤、疼痛类疾病方面,其中27个化合物和20个靶点是网络中的关键节点。僵蚕以代谢通路、神经活性配体-受体相互作用、PI3KAkt信号通路、钙信号通路、cAMP信号通路、癌症通路等为主要作用通路。结论:僵蚕可通过多成分、多靶点、多通路治疗糖尿病、哮喘、肿瘤、疼痛类疾病。

Objective:To discuss main pharmacodynamic mechanism of bombyx batryticatus via the method of network pharmacology.Methods:Chemical database and literature were used to collect chemical components of bombyx batryticatus,TCMSP and Swiss Target Prediction database to obtain the potential targets of compounds,the targets were mapped to the related disease by combining TTD,DisGeNet and other bioinformatics database and modern pharmacological effects of bombyx batryticatus.Cytoscape was used to construct drugcomponent-target-disease network model,David database was applied to analyze the mechanism of KEGG pathway.Results:All 39 chemical components of bombyx batryticatus act on 451 targets,mainly concentrated in diabetes,asthma,cancer and pain diseases,among them,27 compounds and 20 targets are the key nodes in the network.Metabolizing pathway,neuroactive ligand receptor interaction,PI3K-Akt signaling pathway,calcium signaling pathway,cAMP signaling pathway and cancer pathway are the main pathways of Bombyx Batryticatus.Conclusion:Bombyx batryticatus could treat diabetes,asthma,cancer and pain diseases through multiple components,multiple targets and multiple pathways.