α-辅肌动蛋白4和钠氢交换子调节因子1在微电场刺激人宫颈癌Hela细胞迁移中的作用机制

Mechanistic study on the effect ofα-actinin 4 and NHERF1 induced migration of human cervical cancer cells treated by EF

目的探讨α-actinin 4和NHERF1在微电场刺激人宫颈癌Hela细胞迁移运动中的分子机制。方法试验分为阴性对照组(control)、加电组(EF)、NHERF1基因沉默组(siRNA)、基因沉默加电组(siRNA+EF)。(1)RT-qPCR检测各组细胞NHERF1和α-actinin 4 mRNA的表达。(2)划痕试验观察各组细胞迁移速度。结果(1)与对照组比较,EF组NHERF1的表达明显增加;siRNA组和siRNA+EF组NHERF1的表达均明显降低。siRNA+EF组NHERF1的表达比siRNA组稍有增加。EF组α-actinin 4的表达明显减少;siRNA组α-actinin 4的表达明显增加;siRNA+EF组α-actinin 4的表达增加,但差异无显著性。(2)与对照组比较,EF组细胞迁移速度明显增加;siRNA组细胞迁移速度明显降低;siRNA+EF组细胞迁移速度高于siRNA组。结论NHERF1调节α-actinin 4的表达,在微电场刺激人宫颈癌Hela细胞迁移运动中起作用。

Objective To investigate the mechanism ofα-actinin 4 and NHERF1 induced migration of human cervical cancer cells treated by EF.Methods The grouping experiment was divided into control group,EF group,NHERF1 siRNA group,and siRNA+EF group.(1)The expression of NHERF1 andα-actinin 4mrna was detected by RT-qPCR.(2)The cell migration rate was measured by scratch test.Results(1)Compared with the control group,the expression of NHERF1 mRNA increased significantly in EF group,and decreased in siRNA group and siRNA+EF group;the expression of NHERF1 mRNA in siRNA+EF group is slightly higher than that of siRNA group.The expression ofα-actinin 4 mRNA decreased significantly in EF group,and increased in siRNA group;the expression ofα-actinin 4 mRNA in siRNA+EF group is higher,but there was no significant difference.(2)Compared with the control group,the cell migration rate of EF group increased significantly in EF group;the migration rate of cells decreased significantly in siRNA group;the cell migration rate of siRNA+EF group was higher than that of siRNA group.Conclusion NHERF1 regulates the expression ofα-actinin 4,which plays a role in the migration of HeLa cells stimulated by EF.