荷斯坦牛新遗传缺陷单倍型HH7分子筛查

Carrier Screening for Genetic Defect HH7 in Holstein Bulls

HH7(Holstein Haplotype 7)是在荷斯坦牛群中新发现的一种隐性遗传缺陷单倍型,由27号染色体上CENPU基因4个碱基缺失突变引起,隐性基因纯合时能引起胚胎早期流产,严重影响着奶牛养殖者的经济效益。本研究利用竞争性等位基因特异性PCR(KASP法)对随机抽取的166头荷斯坦公牛样本进行了检测分析,结果表明,在所检测的荷斯坦公牛中未发现HH7携带者个体。提示新缺陷单倍型HH7在北京地区的荷斯坦牛群中比例可能很低,但在全国牛群中的分布情况尚不清楚,建议继续开展相关研究,并在种牛遗传物质引进时重点关注,避免将新缺陷基因引入进来,造成有害基因的广泛传播。

Haplotype HH7 is an autosomal recessively inherited disorder recently identified in Holstein cattle.The molecular mechanism of HH7 has been identified as being a 4-bp deletion in CENPU gene downstream the splice donor site at position BTA27.The embryo with homozygous defective alleles will die during early pregnancy,which seriously threatens the economic benefits of dairy farmers.In the present study,a total of 166 randomly selected Holstein bulls were screened for HH7 using Kompetitive Allele-Specific PCR and Sanger sequencing.As a result,no carrier individuals were identified,which is suggested that HH7 has little influence on Chinese Holstein cattle and it can be ignored in the breeding and selection of dairy farms.However,we should pay attention to the introduction of genetic material to avoid an influx of harmful genes.