沙利度胺对于ITP患者Treg及Th17细胞亚群的影响

The effect of thalidomide on Treg and Th17 cell subsets in ITP patients

目的:通过检测沙利度胺治疗前后原发免疫性血小板减少症(primaryimmunethrombocytopenia,ITP)患者外周血调节性T细胞(Treg)与辅助T细胞17(Th17)水平,观察沙利度胺对于ITP患者Treg与T17细胞水平的影响,探讨沙利度胺对于ITP治疗的有效性及可能的理论机制。方法:选择24例初诊ITP患者采用标准治疗方法,20例难治性ITP患者采用沙利度胺治疗,另选取20例健康志愿者,分别采用流式细胞术检测治疗前后外周血Treg与Th17细胞百分率与健康志愿者做对比。结果:ITP患者外周血Treg细胞比例显著低于健康对照组(P<0.0),而Th17细胞比例高于健康对照组,但无统计学意义(P>0.05),沙利度胺治疗后Treg细胞比例较前增加,(P<0.05),Th17细胞无明显变化。结论:沙利度胺可上调难治性ITP患者外周血Treg细胞水平,这可能是沙利度胺治疗ITP有效的机制之一。

Objective:To investigate the effect of thalidomide on the regulatory T cells(Treg)and helper T cells(Th17)levels of primary immune thrombocytopenia patients with peripheral blood Treg and T17 cells before and after thalidomide treated.Methods:24 patients of initial diagnosed ITP were selected with standard treatment,20 patients with refractory ITP were treated with thalidomide,and 20 healthy volunteers were selected.The percentage of Treg and Th17 cells in peripheral blood were compared with volunteers by flow cytometry.Results:the proportion of peripheral blood Treg cells in ITP patients was significantly lower than that in the healthy control group(P<0.05),while the proportion of Th17 cells was higher than that in the healthy control group,but It was no statistical significance(P>0.05).the proportion of Treg cells after thalidomide treated was higher than before(P<0.05),and there was no significant change in Th17 cells.Conclusion:thalidomide upregulates peripheral blood Treg cell levels in patients with refractory ITP,which may be one of the effective mechanisms of thalidomide treatment.