谷氨酸钠摄入引起的果蝇肠道菌群多样性变化研究

Diversity changes of intestinal flora caused by sodium glutamate intake in Drosophila melanogaster

谷氨酸钠(MSG),也称味精,是谷氨酸的钠盐,也是最丰富的天然存在的非必需氨基酸之一。MSG常作为一种增味剂被添加到食物中,但也有报道可引起哮喘、头痛甚至脑损伤。本文以黑腹果蝇作为材料,利用16S rDNA扩增子测序分析其肠道菌群对高MSG摄入的响应。结果发现饲喂MSG组果蝇相比对照组,其肠道的厚壁杆菌门Firmicutes、芽孢杆菌纲Bacilli、乳杆菌目Lactobacillales、乳杆菌科Lactobacillaceae、乳杆菌属Lactobacillus丰度显著升高。α多样性和β多样性分析也显示饲喂MSG组和对照组的微生物群落组成多样性存在显著差异。该研究证明,高MSG摄入能显著改变果蝇肠道菌群组成,为理解高MSG摄入对肠道菌群的影响提供更多的线索和依据。

Monosodium glutamate(MSG),also known as sodium glutamate,is the sodium salt of glutamic acid,one of the most abundant naturally occurring non-essential amino acids.MSG is usually added to food as a flavor enhancer,but it has also been reported to cause asthma,headaches and even brain damage.Here,the 16S rDNA amplicon sequencing was used in Drosophila melanogaster to analyze the changes of gut microbiota in response to high MSG intake.The results showed that MSG intake significantly changed the gut microbiota composition of Drosophila:Compared to the control group,Firmicutes,Bacilli,Lactobacillales,Lactobacillaceae,Lactobacillus abundance were increased significantly in the MSG group.αdiversity andβdiversity analysis also showed significant differences in the composition diversity of the microbial community between the MSG group and the control group.This study proves that high MSG intake significantly changes the gut microbial composition of Drosophila,providing more evidence and clues for understanding the effect of high MSG intake on the intestinal flora.