基于生物信息学的胆囊癌差异表达谱中关键蛋白调控基因分析

Analysis of key protein regulatory genes in differential expression profile of gallbladder cancer based on bioinformatics approaches

背景与目的:胆囊癌(GBC)的机制发生目前仍不清楚,基因组与转录组层面的数据为研究GBC的分子生物学机制提供了基础数据资源。本研究运用生物信息学手段分析GBC组织和胆囊正常组织的差异表达基因以及GBC关键蛋白调控分子,从而探讨GBC发生的潜在分子生物学机制。方法:基于GEO数据库中两个GBC转录组数据集筛选差异表达基因,并对这些基因进行GO三种类型功能注释。利用STRING数据库构建蛋白质互作网络,并进行模块挖掘寻找关键蛋白调控基因。评估关键蛋白调控基因的表达及预测效能。结果:共筛选到140个可重复GBC差异表达基因(上调基因20个,下调基因120个),主要与前脑发育和神经发生的正调控通路密切相关,参与突触后膜和横小管组成。同时发现SFRP1这一关键调控蛋白基因在预测GBC的发生中具有一定作用。结论:本研究获得的GBC转录谱表达的信息可为GBC分子机制的研究提供框架和脉络,关键蛋白调控基因SFRP1可能在GBC的发生与发展中发挥关键生物学作用。

Background and Aims:Th e underlying mechanism for the occurrence of gallbladder carcinoma(GBC)is still unclear at present.Th e available data at the genomic and transcriptomic levels provide the basic data source for investigation of the molecular biological mechanisms of GBC.Th erefore,this study was conducted to to analyze the diff erentially expressed genes in and normal gallbladder tissues and key protein regulatory molecules in GBC by bioinformatics approaches,so as to explore the potential molecular biological mechanism of GBC.Methods:Th e diff erentially expressed genes were screened based on two GBC transcriptional datasets from GEO database,and the three GO functional annotations were performed on these genes.Th e STRING database was applied to construct a protein interaction network,and perform module mining in the investigation of key protein regulatory genes.Finally,the expression and predictive efficacy of the identified key protein regulatory genes were comprehensively evaluated.Results:A total of 140 repeatable differentially expressed genes(20 up-regulated genes and 120 down-regulated genes)in GBC were screened,which are mainly related to the forebrain development and positive regulation of neurogenesis,and participate in the composition of the postsynaptic membrane and transverse tubules.Meanwhile,the SFRP1,a key protein regulatory molecule,had a certain ability in predicting the occurrence of GBC.Conclusion:The information expressed by transcription spectrum of GBC obtained in this study can provide framework and thinking structure for studying the molecular mechanism of GBC.The key protein regulatory molecule SFRP1 probably plays a pivotal role in the occurrence and development of GBC.