摘要
以大豆分离蛋白、麦芽糊精、阿拉伯胶为壁材,利用喷雾干燥法和冷冻干燥法制备核桃油微胶囊。在62℃下将核桃油微胶囊加速氧化14 d,测定过氧化值、茴香胺值及总氧化值。制备模拟肠液和胃液,测定核桃油微胶囊体外模拟消化油脂释放率及游离脂肪酸释放量。选取6~8周龄昆明小鼠,适应性喂养后随机分为8组,分别为空白对照组、高脂模型组、核桃油微胶囊及核桃油低、中、高剂量组,连续灌胃4周,测定其体重、肝脏指数、血液指标、肝脏细胞指标,并对肝脏及腹部脂肪细胞染色观察其损害情况。结果表明:喷雾干燥制备核桃油微胶囊包埋率为81.91%,水分含量为2.40%,流动性高,溶解性较好。62℃加速氧化14 d,微胶囊化可有效缓解核桃油氧化变质,喷雾干燥制备的核桃油微胶囊油脂氧化程度低于冷冻干燥的,且过氧化值、茴香胺值及总氧化值更低。喷雾干燥制备的微胶囊经模拟胃液及肠液消化,油脂释放率高达91.13%,高于冷冻干燥的,游离脂肪酸释放量也更高。喷雾干燥核桃油微胶囊能有效缓解高脂模型小鼠体重及肝脏指数升高,降低血清总胆固醇(TC)、甘油三酯(TG)含量,降低低密度脂蛋白胆固醇(LDL-C)水平、提高高密度脂蛋白胆固醇(HDL-C)水平,提高小鼠肝脏谷胱甘肽过氧化物酶(GSH-PX)水平、超氧化物歧化酶(T-SOD)水平,降低肝脏丙二醛(MDA)水平,从而有效地抑制高脂模型小鼠肝脏脂肪变性和氧化损伤。
Using soybean protein isolate,maltodextrin and arabia gum as wall materials,walnut oil microcapsules was separately prepared by spray drying and vacuum freeze-drying. The walnut oil microcapsules was accelerately oxidized at 62 ℃ for 14 d. The peroxide value,anisidine value and total oxidation value were determined. The simulated intestinal juice and gastric juice were prepared,and the release rates of oil and free fatty acid of walnut oil microcapsules in vitro were determined. Kunming mice aged 6-8 weeks were selected and randomly divided into eight groups after adaptive feeding: blank control group,high-fat model group,walnut oil microcapsules and walnut oil low,medium and high-dose groups. They were administrated continuously for four weeks. Their body weight,liver index,blood index and liver cell index were measured,and the damages of liver and fat cells were observed by staining. The results showed that the encapsulation rate of the walnut oil microcapsuls prepared by spray drying was81. 91%,the moisture content was 2. 40%,the fluidity was high and the solubility was good. Through62 ℃ accelerated oxidation test for 14 d,microencapsulation could effectively alleviate the oxidative deterioration of walnut oil,and the oxidation degree of walnut oil microcapsule prepared by spray drying was lower than that by freeze-drying,and the peroxide value,anisamine value and total oxidation value were lower. The walnut oil microcapsules prepared by spray drying were digested by simulated gastric juice and intestinal juice,and the release rate of oil was up to 91. 13%,higher than that by freeze-drying,and the releasing of free fatty acids was also higher. Walnut oil microcapsule prepared by spray drying could effectively alleviate the increase of body weight and liver index,decrease the contents of serum total cholesterol( TC) and triglyceride( TG),reduce the levels of low density lipoprotein cholesterol( LDL-C),increase the level of high-density lipoprotein cholesterol( HDL-C),increase the level of glutathione peroxidase( GSH-PX) and superoxide dismutase( T-SOD) in liver of mice,and reduce the level of malondialdehyde( MDA) in liver, which could effectively inhibit fatty degeneration and oxidative damage of liver in high-fat mice.
作者
戚登斐
张润光
潘建龙
杨涛
张有林
QI Dengfei;ZHANG Runguang;PAN Jianlong;YANG Tao;ZHANG Youlin(College of Food Engineering and Nutrition Sciences,Shaanxi Normal University,Xi'an 710119,China;Shaanxi Ankang Yingtian Ecological Agriculture and Forestry Development Co.,Ltd.,Ankang 725000,Shaanxi,China)
出处
《中国油脂》
CAS
CSCD
北大核心
2021年第1期24-31,47,共9页
China Oils and Fats
基金
中央高校基本科研业务费资助项目(GK201806002)
陕西省农业科技创新驱动项目(NYKJ-2018-XA-06)
陕西省特色产业创新链-农业领域项目(2018TSCXL-NY-06-02)。
关键词
核桃油
微胶囊
抗氧化性
体外模拟试验
降血脂
护肝功能
walnut oil
microcapsule
antioxidation
in vitro simulation test
blood lipid lowering
liver protection