TAT-XIAP融合蛋白对缺血缺氧新生大鼠远期学习记忆能力的影响

The effect of TAT-XIAP fusion protein on long-term learning and memory impairment in neonatal rats with hypoxic-ischemia

目的探讨TAT-XIAP融合蛋白对缺血缺氧性脑损伤(hypoxic ischemic brain damage,HIBD)新生大鼠生后学习记忆能力的影响。方法将出生7 d的SD大鼠,经氯胺酮腹腔静脉注射麻醉后结扎左颈总动脉,建立HIBD动物模型,通过水迷宫实验筛选出学习记忆障碍大鼠20只,分为TAT-XIAP融合蛋白给药组和对照组,每组10只。TAT-XIAP融合蛋白给药组给予TAT-XIAP大鼠尾静脉注射,对照组给予同体积生理盐水大鼠尾静脉注射,4周后应用水迷宫检测大鼠学习记忆能力。结果游泳轨迹路线多在平台所在象限附近,呈趋向型。与对照组对比,TAT-XIAP融合蛋白给药组的逃避潜伏期时间明显缩短(P<0.05);穿越原平台位置的次数和在原平台象限游程、时间百分比均高于对照组(P<0.05)。结论TAT-XIAP融合蛋白可能改善缺血缺氧新生大鼠生后学习记忆能力。

Objective To investigate the effect of TAT-XIAP fusion protein on long-term learning and memory in neonatal rats with hypoxic-ischemic brain damage(HIBD).Methods An experimental animal model of hypoxic-ischemic brain damage was established by ligating the left common carotid artery after intravenous intraperitoneal injection of ketamine in 50 SD rats born 7 days old.Then 20 rats,screened learning and memory impairment rats by Morris water maze test,were divided into two groups which were TAT-XIAP fusion protein administered group and the control group,10 rats in each group.Rats were received intraperitoneal injection with TAT-XIAP in the treatment of TAT-XTAP group.Control group received the same volume of saline intravenous injection in rats.Morris water maze was used to detect the spatial learning and memory of rats after 4 weeks.Results Most of the swimming trajectory lines of the TAT-XIAP group were near the platform quadrant,which showed a trend-type.The escape latency of Morris water maze test was significantly shorter in TAT-XIAP group than that of control group(P<0.05).In the TAT-XIAP group,the number of times across the original platform position and the original position of the platform in platform quadrant time were increased compared with the model group(P<0.05).Conclusions TAT-XIAP may improve the long-term learning and memory ability of HIBD rats.