基于金纳米簇构建荧光传感方法在生化分析中的应用研究

Study on the Application of Fluorescence Sensor based on Gold Nanoclusters in Biochemical Analysis

近几年来,基于配体保护的金纳米簇的合成工艺迅速发展,采用各种生物和有机化合物合成配体,多种结构性质和功能迥异的金纳米簇被成功制备。并且相较于其他金属纳米簇,金纳米簇在表现出良好生物相容性和物化性质的同时还具有较高的稳定性和合成效率,受到了各个领域的广泛关注,尤其是在化学和生物物质的传感领域。本文综述了采用荧光传感方法,基于金纳米簇检测化学和生物分子的新进展。根据检测对象进行分类,概括分析了几个代表性体系:无机离子、生物活性分子和小分子有机物,并详细阐述了其检测机理、灵敏度及样品检测等结果,同时介绍了各种金纳米簇的合成特点和传感优势。

In recent years,the synthesis of gold nanoclusters based on the protection of ligands has developed rapidly,and many kinds of gold nanoclusters with different structures,properties and functions have been successfully prepared by using various biological and organic compounds to synthesize ligands.Compared with other metal nanoclusters,gold nanoclusters have not only good biocompatibility and physicochemical properties,but also high stability and synthesis efficiency,which have attracted extensive attention in various fields,especially in the field of chemical and biological material sensing.In this review,we focus on the progress of gold nanoclusters'application in the assay of biochemical molecules by adopting fluorescence sensing methods.The main content is classified by three parts based on analyte.The target materials include inorganic ions,bioactive molecules and small molecular organics.For the three representative systems,the detection mechanism,detection results such as sensitivity and samples are illustrated in detail.The synthesis features and sensing advantages of various gold nanoclusters are listed as well.