PPP1R3C基因在3T3-L1前脂肪细胞分化过程中的功能研究

Study on Function of PPP1R3C Gene during Differentiation of 3T3-L1 Preadipocytes

为研究小鼠的蛋白磷酸酶1调节亚基3C(protein phosphatase 1 regulatory subunit 3C,PPP1R3C)基因在脂肪细胞分化中的功能,本研究以小鼠3T3-L1前脂肪细胞为材料,通过基因过表达、干扰及实时定量PCR等实验,分析了PPP1R3C基因对成脂分化的影响,并初步探讨了它对成脂分化影响的机制。结果显示,在3T3-L1前脂肪细胞中,过表达PPP1R3C基因,可促进脂肪细胞分化和成脂标志基因的表达;而敲低PPP1R3C基因的表达,则抑制脂肪细胞的分化和成脂标志基因的表达。进一步研究发现在3T3-L1中敲低固醇调节元件结合转录因子1(sterol regulatory element binding transcription factor 1,SREBP1)基因的表达,则PPP1R3C基因的表达也下调,提示PPP1R3C影响成脂分化可能是通过SREBP1对PPP1R3C的调控来实现的。以上结果表明,PPP1R3C对3T3-L1前脂肪细胞分化具有促进作用,PPP1R3C是一个新的成脂分化调控因子。

To study the function of protein phosphoatase 1 regulatory subunit 3C(PPP1R3C)gene in mouse adipocytes differentiation,using by mouse 3T3-L1 preadipocytes as materials,we analyzed the effect of PPP1R3C gene on adipogenic differentiation through gain of function and loss of function experiments,and we preliminarily investigated the mechanism of its effect on adipogenic differentiation.The results showed that PPP1R3C was overexpressed in 3T3-L1 cells,which promoted adipocyte differentiation and up-regulated the expression of adipocyte marker genes.Knocking down the expression of PPP1R3C gene inhibited the differentiation of adipocytes and the expression of adipogenic differentiation marker gene.Further studies found that knocking down the expression of sterol regulatory element binding transcription factor 1(SREBP1)gene in 3T3-L1 cells resulted in down-regulation of PPP1R3C gene expression,suggesting that the influence of PPP1R3C on adipogenic differentiation may be mediated through regulation of SREBP1.It can be concluded that PPP1R3C promotes the differentiation of 3T3-L1 preadipocytes,and PPP1R3C is a new regulator of adipogenic differentiation during the differentiation of 3T3-L1 preadipocytes.