双重超声造影评估胃癌细胞侵袭及血管生成的价值

Value of double contrast-enhanced ultrasonography in evaluating invasion and angiogenesis of gastric cancer cells

目的探讨双重超声造影评估胃癌患者癌细胞增殖、侵袭功能及血管新生的临床应用价值。方法选取我院收治的40例胃癌患者为观察组,另选同期行胃镜检查的健康成人50例为健康对照组。使用双重超声造影检测两组增强时间(ET)、峰值时间(TTP)及曲线上升支斜率(WIR),比较胃癌细胞侵袭基因表达量和血管新生指标水平,分析超声造影参数与胃癌细胞侵袭及血管新生指标的相关性。结果观察组患者ET、TTP、E-钙黏蛋白(E-cadherin)水平均低于健康对照组,WIR、真核起始因子4E(EIF4E)、CD44基因、环指域蛋白1(UHRF1)、内皮生长因子(VEGF)、促生长因子(IGF-1)、α-1-微球蛋白-胰蛋白酶抑制剂前体(AMBP)、可溶性E-钙黏蛋白(SE-CAD)水平均高于健康对照组,差异均有统计学意义(均P<0.05)。相关性分析显示,ET、TTP与E-cadherin均呈正相关,与EIF4E、CD44、UHRF1、VEGF、IGF-1、AMBP、SE-CAD均呈负相关(均P<0.001);WIR与E-cadherin呈负相关,与EIF4E、CD44、UHRF1、VEGF、IGF-1、AMBP、SE-CAD均呈正相关(均P<0.001)。结论双重超声造影能准确评估胃癌患者癌细胞侵袭和血管生成,具有较好的临床应用价值。

Objective To investigate the clinical application value of double contrast-enhanced ultrasound in evaluating the proliferation,invasion and angiogenesis of cancer cells in gastric cancer patients.Methods Forty patients with gastric cancer admitted in our hospital were selected as the observation group,and 50 healthy patients with simultaneous gastroscopy were taken as the control group.The enhancement time(ET),time-to-peak(TTP)and wash-in-rate(WIR)were measured by double contrast-enhanced ultrasound,the expression of invasion gene and angiogenesis index of gastric cancer cells were compared,and the correlation between contrast-enhanced ultrasound parameters and invasion and angiogenesis index was analyzed.Results The levels of ET,TTP and E-cadherin in the observation group were significantly lower than those in the healthy control group,while WIR and the levels of eukaryotic initiation factor 4e(EIF4E),CD44,ring finger domain protein 1(UHRF1),endothelial growth factor(VEGF),growth-promoting factor(IGF-1),α-1-microglobulin-trypsin inhibitor precursor(AMBP)and soluble E-cadherin(SE-CAD)in the observation group were significantly higher than those in the healthy control group(all P<0.05).Correlation analysis showed that ET and TTP were positively correlated with E-cadherin,and negatively correlated with EIF4E,CD44,UHRF1,VEGF,IGF-1,AMBP,SE-CAD(all P<0.001),WIR was negatively correlated with E-cadherin,and positively correlated with EIF4E,CD44,UHRF1,VEGF,IGF-1,AMBP,SE-CAD(all P<0.001).Conclusion Double contrast-enhanced ultrasound can accurately evaluate the invasion and angiogenesis of cancer cells in gastric cancer patients,which has good clinical application value.