流式细胞术在眼部疾病细胞因子和细胞学检查中的应用进展

Application progress of flow cytometry in the cytokine test and cytological examination for ocular diseases

流式细胞术是一种能够对单个细胞或生物颗粒进行多参数、定量分析和分选的检测技术,是近年来迅速发展起来的一项先进的细胞定量分析技术,广泛应用于免疫学、肿瘤学和血液学等多领域医学研究和临床诊断。该技术可以定量检测和分析眼内液中细胞因子水平及各细胞亚群的分布特征。与传统的酶联免疫吸附试验、细胞形态学检查及免疫组织化学检测等方法比较,流式细胞术具有操作简单、所需样本量小、灵敏度高、通量高等优点。流式细胞术已广泛应用于眼内新生血管相关疾病、眼内淋巴瘤、视网膜病变、白内障、葡萄膜炎、结节病性葡萄膜炎、感染性眼内炎症性疾病等眼部疾病相关细胞因子水平检测和细胞亚群分析中,对眼部疾病的发病机制研究和靶向治疗起到日益重要的作用。本文就流式细胞术在眼部疾病眼内液的细胞因子和细胞学检查中的应用进展进行综述。

As an advanced quantitative technology for cells,flow cytometry,which can make multi-paramter quantitative analysis and sorting of individual cells or biological particles,has been widely used in immunology,oncology,hematology and other fields of medical research and clinical diagnosis in recent years.It can quantitatively detect the expression levels of cytokines and the distribution characteristics of cell subsets in the intraocular fluid.Compared with traditional enzyme-linked immunosorbent assay,cell morphology and immunohistochemistry,it has the advantages of simple operation,smaller sample size,higher sensitivity and higher throughput.Flow cytometry has been widely used in the detection and subgroup analysis of cytokines related to eye diseases,such as intraocular angiogenesis,intraocular lymphoma,retinopathy,cataract,uveitis,sarcoidotic uveitis,infectious intraocular inflammation,etc.Flow cytometry plays an increasingly important role in the pathogenesis research and targeted therapy of eye diseases.In this article,the application of flow cytometry in the examination of cytokines and cytology in ocular fluid for ocular diseases were reviewed.