红茶菌中细菌纤维素产生菌的筛选、鉴定及其发酵动力学模型构建

Screening,identification of bacterial cellulose producing bacteria and establishment of fermentation kinetics

从红茶菌中筛选获得细菌纤维素(bacterial cellulose,BC)产生菌株C 1,结合形态学特征及分子生物学手段,将其鉴定为木糖驹形氏杆菌(Komagataeibacter xylinus)。随后以筛选所得菌株C 1为试验菌,以葡萄糖为碳源,通过分批发酵制备BC,分别构建菌体生长、产物生成以及底物消耗的动力学模型,并确定模型的动力学参数。结果表明,细菌纤维素的合成属于部分生长偶联型,3种模型的相关系数R 2分别为0.998、0.9589和0.9623,说明实验值与模型值拟合良好,建立的动力学模型能够较好的预测木糖驹形氏杆菌发酵产BC过程中各参数的变化。该研究可以为BC的精准发酵提供技术支撑。

A bacterial cellulose(BC)producing strain C 1 was screened from Kombucha and identified as Komagataeibacter xylinus,based on morphological characteristics and molecular biology.Then,the obtained strain C 1 was used as the test bacterium to ferment BC,using glucose as carbon source.Kinetic models of bacterial growth,BC production,and substrate consumption were constructed,respectively,and the kinetic parameters of the model were determined.The results showed that the synthesis of BC belonged to the type of partial growth coupling,and the correlation coefficients R 2 of the three models were 0.998,0.9589,and 0.9623,respectively,indicating that the experimental values fit well with the model,and the established kinetic model can better predict the parameters in the fermentation process of Komagataeibacter xylinus.This study provides technical support for the precise fermentation of BC.