云南边贸进口即食食品中金黄色葡萄球菌的分离与鉴定

Identification and isolation of Staphylococcus aureus in ready-to-eat food imported from Yunnan border trade

为探索云南边贸进口即食食品中金黄色葡萄球菌的污染状况,对云南5个边境口岸城市采集到的10类459份进口边贸即食食品中的金黄色葡萄球菌进行检验和分离,并对分离出的可疑金黄色葡萄球菌进行生化确认、全自动微生物鉴定以及16S rDNA序列鉴定,同时利用16S rDNA序列构建系统发育树和同源性分析。结果表明,从所采集的样品中检出2份阳性食品,检出率为0.43%,均为糖果和巧克力,占该类食品的3.8%。从2份阳性食品中分离出9株金黄色葡萄球菌菌株,通过16S rDNA系统发育树和遗传进化分析,9株金黄色葡萄球菌不存在明显的样品来源不同遗传进化差异。该研究结果为构建云南边贸进口食品食源性致病菌溯源技术、致病菌安全预警平台提供理论和技术支持。

In order to investigate the contamination of Staphylococcus aureus in the imported ready-to-eat foods from border trade in Yunnan province,459 samples of 10 categories collected from 5 border port cities were examined for S.aureus.Biochemical identification,automatic microbial identification(VITEK)and 16S rDNA sequence identification were performed for the isolation of suspicious S.aureus.At the same time,the phylogenetic tree and homology analysis were constructed by using 16S rDNA sequence.The results showed that the detection rate of S.aureus was 0.43%,all of which were candy and chocolate,accounting for 3.8%of this category.Nine strains of S.aureus were isolated from two positive foods,according to phylogenetic analysis of 16S rDNA phylogenetic tree and genetic evolution,these S.aureus strains did not show significant genetic and evolutionary differences in different sample sources.The results provide theoretical and technical support for the establishment of foodborne pathogenic bacteria tracing technology and pathogenic bacteria safety-warning platform for border trade food import in Yunnan province.