微生物嗜热脂肪酶研究进展

Research progress on microbial thermophilic lipase

微生物发酵制备脂肪酶是工业化生产脂肪酶的主要途径。由于工业化生产对脂肪酶的热稳定要求较高,野生菌来源的嗜热脂肪酶已无法满足工业化的高需求。来源于微生物的脂肪酶在工业中的应用逐渐成为生物工程研究的热点,构建高表达且酶表征好的产酶菌株是目前研究的重点。因此,该文就近年来嗜热脂肪酶产生菌及其表征、异源表达系统、结构改造、分离纯化和固定化等方面研究进展进行综述,重点阐述突出的成果,为该方向联用多个分子生物技术的研究提供理论参考和方法借鉴。

Microbial fermentation is the main way of industrial production of lipase.Thermophilic lipase derived from wild bacteria could not meet the requirement of thermal stability of lipase for industrial production.The application of microbial lipase in industry has gradually become the focus of bioengineering research,and the construction of high-yield and well-characterized enzyme-producing strains is the focus of current research.Therefore,in this paper,the recent research progress on the thermophilic lipase involving the producing bacteria and enzymatic characterization,the heterologous expression system,structural modification,isolation,purification and immobilization and so on were reviewed,and the outstanding achievements were mainly described,so as to provide theoretical and methodological references for the research in this direction.