荧光法测定饮料中邻苯二甲酸二乙酯的含量

Determination of Diethyl Phthalate in Beverages by Fluorescence Spectrophotometry

塑化剂在塑料材料、食品包装材料、医用材料、个人护理用品等数百种产品中广泛应用。建立了一种简便快速并适用于测定饮料中邻苯二甲酸二乙酯(DEP)的方法。DEP能够与罗丹明B形成配合物,使罗丹明B的荧光强度增强。实验结果表明,在pH值7的PBS缓冲溶液中,20℃温度下,SDBS的浓度8×10^(-5)mol/L,PBS用量2 mL,罗丹明B的浓度8×10^(-6)mol/L。方法的线性范围为4.0×10^(-6)~2.0×10^(-5)mol/L,相对标准偏差为2.3%~3.8%,加标回收率为103%~108%。

Plasticizers are widely used in hundreds of products such as plastic materials,food packaging materials,medical materials,and personal care products.A rapid and sensitive method for the determination of diethyl phthalate(DEP)in beverages is reported.DEP can form a complex with Rhodamine B and increase the fluorescence intensity of Rhodamine B.The experimental results show that the optimal conditions are pH 7,temperature 20℃,SDBS concentration 8×10^(-5)mol/L,PBS dosage 2 mL,and Rhodamine B concentration 8×10^(-6)mol/L.The linear range of the method is 4.0×10^(-6)~2.0×10^(-5)mol/L.The relative standard deviation is 2.3%~3.8%,and the recoveryis 103%~108%.