分子印迹阵列固相微萃取用于检测果蔬中多种有机磷农药残留

Determination of Multiple Organophosphorus Pesticide Residues in Fruits and Vegetables by Molecularly Imprinted Array Solid Phase Microextraction

分别以水胺硫磷、二嗪农为模板分子,采用溶胶-凝胶法制备分子印迹固相微萃取头,以2种萃取头各两根组装成分子印迹阵列固相微萃取装置,建立顶空固相微萃取与气相色谱联用的方法用于检测果蔬中5种有机磷农药残留量。阵列萃取方法的检出限为0.0028~0.0436μg/kg,低于单根分子印迹固相微萃取法。采用该阵列固相微萃取装置对果蔬样品中的农药残留量进行分析,苹果和马铃薯样品中5种分析物的加标回收率均在78.7%~122.8%之间。研究表明,分子印迹阵列固相微萃取装置萃取率高、灵敏度高、准确度高、选择性好、分析通量大,适用于复杂体系中多目标物的同时分析测定。

Two types of molecularly imprinted solid phase microextraction fibers were fabricated through sol-gel method using diazinon and isocarbophos as the templates,respectively,and were assembled together to constitute a molecularly imprinted array solid phase microextraction device.The device was then used in combination with gas chromatography for the determination of five organophosphorus pesticide residues in fruits and vegetables.The proposed method exhibited very low limits of detection,ranging from 0.0028 to 0.0436μg/kg,lower than those obtained by using a single molecularly imprinted solid phase microextraction fiber.The recoveries of five analytes in spiked apple and potato samples were 78.7%–122.8%.The study showed that the molecularly imprinted array solid phase microextraction device had the advantages of high extraction efficiency,high sensitivity,good accuracy and selectivity,and high throughput,and could be suitable for simultaneous determination of multiple analytes in complex matrices.