两种乳酸杆菌对肥胖小鼠的干预作用

Intervention Effect of Two Lactobacillus Strains on Obesity in Mice

目的:探讨两种乳酸杆菌对高脂饮食诱导的肥胖小鼠的干预作用。方法:将72只C57BL/6J雄性小鼠随机分成6组,分别为普通饲料组、高脂饲料组、高脂饲料和Lactobacillus casei NCU011054低剂量组、高脂饲料和Lactobacillus casei NCU011054高剂量组、高脂饲料和Lactobacillus acidophilus NCU433低剂量组、高脂饲料和Lactobacillus acidophilus NCU433高剂量组。每周对小鼠称体质量,并在第9周末(动物实验结束)处死小鼠,测定小鼠空腹血糖浓度、Lee’s指数、器官指数及器官功能指标水平、血清生化指标水平、血清瘦素和脂联素质量浓度、肝脏氧化应激指标水平、肝脏胆固醇(cholesterol,TC)和甘油三酯(triglyceride,TG)含量,制作脂肪组织病理切片观察脂肪细胞形态和分化情况,检测小鼠粪便中短链脂肪酸的含量。结果:两株乳酸杆菌显著降低了小鼠体质量增量、Lee’s指数、空腹血糖浓度以及血清中TC、TG、低密度脂蛋白胆固醇水平和血清瘦素水平(P<0.05),并显著升高了血清中高密度脂蛋白和脂联素水平(P<0.05)。此外,乳酸杆菌的摄入有效抑制了肥胖小鼠脂肪细胞的分化并改善了肝脏脂代谢紊乱。结论:两株乳酸杆菌对肥胖小鼠具有有效的干预作用,其能够通过调节小鼠体内脂类相关水平的变化发挥作用。

Purpose:To study the intervention effect of two Lactobacillus strains on obesity induced by a high-fat diet in mice.Methods:Totally 72 male C57BL/6J mice were randomly divided into six groups:normal diet,high-fat diet,high-fat diet plus low-dose Lactobacillus casei NCU011054,high-fat diet plus high-dose Lactobacillus casei NCU011054,high-fat diet plus low-dose Lactobacillus acidophilus NCU433,and high-fat diet plus high-dose Lactobacillus acidophilus NCU433 groups.The mice were weighed weekly,and sacrificed at the end of the nine-week experimental period.Fasting blood glucose(FBG),Lee’s index and visceral organ indexes were measured,and serum biochemical indicators,serum leptin and adiponectin(ADPN)levels were determined.Oxidative stress indicators,cholesterol(TC)and triglyceride(TG)contents in liver and short chain fatty acid content in mice feces were measured,and the morphology and differentiation of adipose tissue were examined.Results:Both Lactobacillus strains not only significantly reduced the body mass gain,Lee’s index and FBG level of obese mice as well as the levels of TC,TG,and low-density lipoprotein cholesterol(LDL-C)in the serum(P<0.05),but also significantly elevated serum high-density lipoprotein cholesterol(HDL-C)and ADPN levels(P<0.05).Moreover,Lactobacillus treatment suppressed the differentiation of adipocytes and ameliorated lipid metabolism disorders in the liver of obese mice.Conclusion:Both Lactobacillus strains effectively regulate lipid metabolism in obese mice.