绵羊GnRHR基因生物信息学分析

Bioinformatic Analysis of Sheep(Ovis aries)Gn RHR Gene

促性腺激素释放激素受体(gonadotropin releasing hormone receptor,GnRHR)是绵羊(Ovis aries)繁殖性状相关的主效基因,基因结构复杂。本研究以GnRHR基因序列及编码蛋白序列为材料,通过生物信息学方法分析了GnRHR基因的DNA序列、启动子及CpG岛、RNA结构及该蛋白的理化性质、亚细胞定位、跨膜区域、互作蛋白、系统发育等。结果表明,绵羊GnRHR位于6号染色体,潜在核心启动子在1124~1174 bp,没有CpG岛。GnRHR基因编码328个氨基酸残基,其分子量为34252.82 Da,Leu(亮氨酸)所占比例最高,为14.94%;该蛋白定位于内质网,有7个跨膜结构区域;主要的二级结构元件是α-螺旋结构和无规卷曲,包含一个SAM-dependent MTase功能结构域,同时有10个可能的互作蛋白;进化分析表明牛与绵羊GnRHR蛋白亲缘关系最近。本研究结果为进一步深入研究GnRHR的具体基因功能和对绵羊繁殖性能的研究提供帮助。

Gonadotropin releasing hormone receptor(GnRHR)is the main gene related to the reproductive characters of sheep,and the gene structure is complex.The nucleic acid sequence and amino acid sequence of GnRHR gene were analyzed and predicted by bioinformatics methods,including DNA sequence,promoter and CpG island and RNA structure,physicochemical properties,the subcellular position,transmembrane region,protein interaction networks and evolutionary tree.The results showed that GnRHR was located on chromosome 6.The potential core promoter was located at 1124 bp^1174 bp and no CpG island was predicted.The GnRHR gene encodes328 amino acid residues with a molecular weight of 34252.82 Da,Leu(Leucine)accounted for the highest proportion,14.94%.The protein is more likely localized in the cytoplasm with 7 transmembrane structure.The major secondary structure elements is an a-helical structure and a random coil,which contains a SAM-dependent MTase functional domain with 10 interacting proteins.Phylogenetic analysis shows that Bos taurus have the closest relationship with Ovis aries GnRHR protein.The results provide certain help for further research on the specific gene function of GnRHR and the reproductive performance of sheep.