水稻OsCAT2的Crispr/cas敲除植株的全基因表达谱分析

Analysis of the Whole Gene Expression Profile of Os CAT2 Crispr/cas Knockout Plant by Gene Chip

为了进一步了解水稻过氧化氢酶基因OsCAT2的分子机制及其调控网络,本研究以野生型及OsCAT2基因的Crispr/cas转基因敲除植株为材料进行基因芯片分析。通过差异基因的筛选共获得5483个差异表达基因,其中3248个上调表达,2235个下调表达。通过GO功能注释发现,差异基因在二价金属离子运输、锌-Ⅱ离子运输、次生代谢产物生物合成过程和二价无机阳离子转运代谢途径中显著富集;通过KEGG通路分析发现,差异基因在次生代谢产物的生物合成、植物-病原互作、光合作用、氰基氨基酸代谢和类黄酮生物合成途径中显著富集。进一步分析发现,差异表达基因中还包括一系列与植株分蘖数及抗性相关的基因。该研究为深入探讨水稻过氧化氢酶基因OsCAT2的作用机制提供了理论参考。

In order to further understand the molecular mechanism and regulatory network of rice catalase gene OsCAT2,the wild type and the OsCAT2 Crispr/cas transgenic knockout plants were used for gene chip analysis.A total of 5483 differentially expressed genes were obtained,of which 3248 were up-regulated and 2235 were down-regulated.Through GO functional notes,it was found that differentially expressed genes were significantly enriched in bivalent metal ion transport,zinc-Ⅱ ion transport,secondary metabolite biosynthesis and bivalent inorganic cation transport metabolic pathways.The KEGG pathway analysis indicated that differentially expressed genes were obviously enriched in secondary metabolite biosynthesis,plant-pathogen interaction,photosynthesis,cyano amino acid metabolism and flavonoids biosynthesis pathway.Further analysis showed that a series of genes related to tiller number and resistance were also included in the differentially expressed genes.This study provides a theoretical reference for further study the mechanism of rice OsCAT2 gene.