金针菇小G蛋白家族基因鉴定及表达分析

Characterization and Expression Analysis of Small GTPases Families in Flammulina velutipes

小G蛋白在真核生物的生长发育和环境响应中起着重要作用。本研究在可结实大型真菌金针菇(Flammulina velutipes)的基因组序列中,鉴定出了34个小G蛋白基因。其中,金针菇的34个小G蛋白可归类到5个家族:7个属于Ras家族,7个属于Rho家族,7个属于Arf家族,12个属于Rab家族,1个属于Ran家族。结合构巢曲霉(Aspergillus nidulans)、新型隐球菌(Cryptococcus neoformans)、稻瘟病菌(Magnaporthe oryzae)、酿酒酵母(Saccharomyces cerevisiae)和草菇(Volvariella volvacea)的小G蛋白序列,对金针菇小G蛋白家族进行系统发育分析。结果显示:真菌的小G蛋白家族比较保守,Fv-Rho6与草菇线粒体小G蛋白Rho具有较高同源性,属于线粒体小G蛋白。使用金针菇不同发育时期的转录组数据对该家族的基因表达进行分析,结果表明:小G蛋白的表达可聚类为7种表达模式,并且具有明显的组织特异性。本研究可以为解析大型可结实真菌的生长发育机制提供数据参考。

Small GTPases play important roles in the growth,development and environmental responses of eukaryotes.In the genomic sequence of the mushroom forming fungus Flammuliua velutipes,34 small GTPases were identified in this study.Small GTPases in F.velutipes can be grouped into five families:seven are in the Ras family,seven are in the Rho family,12 are in the Rab family,one is in the Ran family and seven are in the Arf family.Phylogenetic analysis of small GTPases of Aspergillus nidulans,Cryvtococcus neoformans,Magnaporthe oryzae,Saccharomyces cerevisiae and Volvariella volvacea revealed that Fv-Rho6 was grouped with GTPase peripheral to the mitochondrial inner membrane in the outgroup.Analysis based on transcriptomic data suggested that the expression of small GTPases has tissue specificity.Seven expression patterns of these small GTPases were also identified during different developmental stages.Overall,this study could be helpful to the growth mechanism in mushroom-forming fungi.