摘要
Desirable scaffolds for tissue engineering should be biodegradable carriers to supply suitablemicroenvironments mimicked the extracellular matrices for desired cellular interactions and to providesupports for the formation of new tissues. In this work, a kind of slightly soluble bioactiveceramic akermanite (AKT) powders were aboratively selected and introduced in the PLGA matrix,a novel L-lactide modified AKT/poly (lactic-co-glycolic acid) (m-AKT/PLGA) composite scaffold wasfabricated via a solvent casting-particulate leaching method improved by solvent self-proliferatingprocess. The effects of m-AKT contents on properties of composite scaffolds and on MC3T3-E1 cellularbehaviors in vitro have been primarily investigated. The fabricated scaffolds exhibited threedimensionalporous networks, in which homogenously distributed cavities in size of 300–400 lmwere interconnected by some smaller holes in a size of 100–200 lm. Meanwhile, the mechanicalstructure of scaffolds was reinforced by the introduction of m-AKT. Moreover, alkaline ionic productsreleased by m-AKT could neutralize the acidic degradation products of PLGA, and the apatitemineralizationability of scaffolds could be largely improved. More valuably, significant promotionson adhesion, proliferation, and differentiation of MC3T3-E1 have been observed, which implied thecalcium, magnesium and especially silidous ions released sustainably from composite scaffoldscould regulate the behaviors of osteogenesis-related cells.
基金
This work was supported by the National Nature Science Foundation of China(Project no.51202151).
