舒马栎CHLD基因克隆及其表达分析

Cloning and Expression Analysis of CHLD Gene in Shumard Oak(Quercus shumardii Buckley)

叶绿素是植物绿叶的主要呈色物质,镁离子螯合酶是叶绿素生物合成途径中的一个关键酶,对叶片的呈色具有重要影响。本研究从舒马栎叶片中克隆了一个镁离子螯合酶ChlD亚基(CHLD)基因,该基因cDNA全长为2851 bp,具有完整的开放阅读框,编码804个氨基酸,与其他植物序列具有较高的一致,故命名为QsCHLD。生物信息学分析显示,QsCHLD基因编码的蛋白无信号肽,具有1个跨膜结构域和多个磷酸化位点。基因表达水平检测结果表明,QsCHLD基因在舒马栎绿色叶片中的表达水平显著高于黄色叶片。这一结果为今后通过基因工程手段调控舒马栎叶片呈色提供了科学依据。

Chlorophyll is the main coloring substance of plant green leaves,and magnesium-chelatase is a key enzyme in the chlorophyll biosynthetic pathway,which plays an important role in leaf coloration.In this study,a magnesium ion chelatase ChlD subunit(CHLD)gene has been cloned from shumard oak(Quercus shumardii Buckley)leaves,it has a full-length cDNA of 2851 bp with a complete open reading frame encoding 804 amino acids.It has high identity with other plant sequences,so it is named QsCHLD.Bioinformatics analysis revealed that the protein encoded by QsCHLD gene has a transmembrane domain and multiple phosphorylation sites,but no signal peptide.The results of gene expression detection showed that the expression level of QsCHLD gene in the green leaves of shumard oak was significantly higher than that in yellow leaves.This result laid the foundation for regulating leaf color of shumard oak by genetic engineering in the future.