摘要
为了研究砂藓(Racomitrium canescens)中的小G结合蛋白基因RcRab在非生物胁迫下发挥的作用,以砂藓为材料,利用实时荧光定量PCR,对砂藓的RcRab基因的表达情况进行了分析。结果表明,RcRab在不同的逆境胁迫处理下的表达不相同;与对照相比,RcRab在干旱和高温处理下表达量均升高,在低温和水渍处理下表达量先下降再上升,说明RcRab基因在多重胁迫下发挥重要的调控作用。将RcRab基因成功构建到植物表达载体pRI101-AN上,为进一步研究其抗逆功能奠定基础。
In order to explore the abiotic stress expression of RcRab gene in Racomitrium canescens,taken Raco-%mitrium canescens as material,the expression of RcRab was detected by real-time fluorescence quantitative PCR.The results showed that the expression of was different in different stress conditions.Compared to the control,the expression of RcRab was increased under drought and high temperature,while it was declined under cold temperature and waterlogging treatment.These results suggested that the RcRab gene might play imporant regulatory roles under a variety of stresses.The RcRab gene was constructed to plant expression vector of pRI101-AN,which may provide a way towards the elucidation of the function.
作者
孙苗龙
柴媛媛
刘博
陈洪
沙伟
钱朋智
张梅娟
SunMiaolong;Chai Yuanyuan;Liu Bo;Chen Hong;ShaWei;Qian Pengzhi;Zhang Meijuan(Key Laboratory of Resistance Gene Engineering and Preservation of Biodiversity in Cold Areas,College of Life Sciences and Agriculture and Forestry,Qiqihar University,Qiqihar,161006;College of Food and Bioengineering,Qiqihar University,Qiqihar,161006)
出处
《分子植物育种》
CAS
北大核心
2021年第5期1546-1550,共5页
Molecular Plant Breeding
基金
齐齐哈尔大学2019年大学生创新创业训练计划项目(201910232083)
黑龙江省省属高等学校基本科研业务费科研项目(135209262,135309459)
黑龙江省省属高等学校基本科研业务费科研项目(YSTSXK201834)
齐齐哈尔市科学技术计划项目(NYCG-201912)共同资助。
