E_(2)通过p38 MAPK及PI3K/Akt信号通路调控奶牛子宫内膜上皮细胞中COX-2的表达

E_(2)Regulates the Expression of COX-2 in Endometrial Epithelial Cells of Cows Through p38 MAPK and PI3K/Akt Signaling Pathway

为了探究p38 MAPK及PI3K/Akt信号通路是否参与雌二醇(E_(2))对奶牛子宫内膜上皮细胞中COX-2的表达及PGF_(2α)分泌的调节,通过体外培养妊娠早期奶牛子宫内膜上皮细胞,并添加E_(2)单独或联合p38 MAPK抑制剂SB203580和PI3K抑制剂LY294002处理奶牛子宫内膜上皮细胞。通过Western Blot、qRT-PCR检测COX-2基因和蛋白的表达,ELISA技术检测PGF_(2α)的水平变化。结果显示:E_(2)促进了妊娠早期奶牛子宫内膜上皮细胞中COX-2的表达和PGF_(2α)的分泌;与此同时,E_(2)激活了p38 MAPK及PI3K/Akt信号通路,添加SB203580和LY294002则抑制了E_(2)对COX-2表达及PGF_(2α)分泌的促进作用。说明E_(2)通过激活p38 MAPK及PI3K/Akt信号通路促进了COX-2的表达及PGF_(2α)的分泌。

To investigate whether p38 MAPK and PI3K/Akt signaling pathways are involved in the regulation of estradiol(E_(2))on PGF_(2α)secretion in cow endometrial epithelial cells,endometrial epithelial cells of pregnant dairy cows were cultured in vitro,and different concentrations of E_(2)and p38 MAPK and PI3K/Akt pathway inhibitors SB 203580 and LY294002 were used to treat cows′endometrial epithelial cells respectively.The expression of COX-2 gene and protein and the level of PGF_(2α)were detected by Western Blot,qRT-PCR and ELISA.The results showed that E_(2)promoted the expression of COX-2 and the secretion of PGF_(2α).In addition,E_(2)activated the p38 MAPK and PI3K/Akt signaling pathways.Moreover,the addition of SB203580 and LY294002 inhibit the promotion effect of E_(2)on COX-2 expression and PGF_(2α)secretion.Therefore,it proved that E_(2)promoted the expression of COX-2 and the secretion of PGF_(2α)by activating the p38 MAPK and PI3K/Akt signaling pathways.