哺乳动物细胞无血清全悬浮培养技术研究进展

Research Progress on Serum-free Suspension Culture of Mammalian Cells

随着生物制品行业的快速发展,哺乳动物细胞无血清全悬浮培养技术已经运用到越来越多的领域中。该技术能够扩大哺乳动物细胞培养的规模,提高生物制品的产量及质量,拓宽哺乳动物细胞的应用领域,并促进哺乳动物细胞生产实现工业化与产业化。但悬浮驯化后的细胞,与父母代细胞相比,蛋白表达水平与生长调节通路均发生了不同程度的改变,使其生产性能受到影响。对此,国内外学者开展了大量的研究并取得了较好进展。作者阐述了哺乳动物细胞无血清全悬浮培养技术的研究现状以及临床生产工艺的优化策略,以期为哺乳动物细胞无血清全悬浮培养技术的发展提供参考。

With the rapid development of biological products industry,the serum-free suspension culture technology of mammalian cells has been involved in more and more fields.This technology can expand the scale of mammalian cells,improve the production and quality of biological products,broaden the application field of mammalian cells,and promote industrialized production of mammalian cells.However,compared with the parent cells,the protein expression level and growth regulation pathway of the cells after suspension acclimation have changed to varying degrees,which affected the production performance of cell lines.In this regard,scholars at home and abroad have carried out a lot of research and made good progress.In this paper,the research status of mammalian cells serum-free suspension culture technology and the optimization strategy of clinical production process were descried,in order to provide references for the development of mammalian cells serum-free suspension culture technology.