大孔树脂精制荷叶黄酮的工艺研究

Research on the technology of refining flavonoidfrom lotus leaf by macroporous adsorption resin

从荷叶中提取黄酮类化合物,并对11种大孔吸附树脂的静态吸附效果进行了比较,选出最优树脂HPD-100。通过研究温度、酸度对HPD-100吸附能力的影响以及不同乙醇体积分数对该树脂的解吸作用,确定分离纯化荷叶总黄酮的新工艺。结果表明:HPD-100对荷叶黄酮的吸附能力最强,其在温度25℃、pH 3.0时吸附效果最好,当乙醇体积分数为70%时,洗脱效果最好,荷叶黄酮纯度可达55.28%。

Flavonoids were extracted from lotus leaves,and the HPD-100 was choosen by comparing absorption effects of 11 kinds of macroporous resin.A new process for the separation and purification of total flavonoids from lotus leaves was determined by studing the effects of temperature and acidity on the adsorption capacity of HPD-100 and the desorption of different volume fraction of ethanol on the resin.The results showed that HPD-100 had the greatest absorption ability at pH 3°and 25℃.When the volume concentration of alcohol was 70%,the desorption was most effective and the purity of flavonoids could reach up to 55.28%.