ALA-PDT通过NF-kB通路调节巨噬细胞极化状态

AlA-PDT regulates macrophage polarization through the NF-KB pathway

目的:探讨ALA-PDT对巨噬细胞极化的影响,并探讨相关机制。方法:采用免疫荧光、PCR、WB等试验检测巨噬细胞极化特征蛋白CD86、CD206、iNOS、ARG-1的表达水平变化,NF-kB激活-核转运检测试剂、WB试验检测NF-kB通路激活情况,WB试验检测抑制NF-kB通路后CD86、iNOS蛋白表达水平。结果:免疫荧光、PCR、WB实验结果显示,PDT处理后,巨噬细胞M1特征蛋白CD86、iNOS表达水平增高,M2特征蛋白表达水平下降(P<0.05)。NF-kB激活-核转运检测、WB实验结果表明PDT处理后,NF-kB通路被激活。给予NF-k B通路抑制剂处理,PDT处理后巨噬细胞M1特征蛋白CD86、iNOS表达水平增高现象被抑制。结论:ALA-PDT通过激活NF-kB通路促进巨噬细胞向M1极化。

Objective: To investigate the effect of ALA-PDT on macrophage polarization and its related mechanism.Methods: Immunofluorescence,PCR,WB and other tests were used to detect the expression levels of CD86,CD206,i NOS and ARG-1,NF-k B activation-nuclear transfer detection reagent and WB test were used to detect the activation of NF-kB pathway and the expression levels of CD86 and i NOS after the inhibition of NF-kB pathway by WB test. Results: Immunofluorescence,PCR and WB showed that the expression levels of CD86 and i NOS of M1-characteristic protein in macrophages increased,while the expression levels of M2-characteristic protein decreased after the treatment of PDT( P <0. 05). The NF-kB activation-nuclear transfer test results showed that the NF-k B pathway was activated after the treatment of PDT. After treatment with the NF-k B pathway inhibitor,the expression levels of CD86 and i NOS of macrophage M1 characteristic proteins were inhibited after PDT treatment. Conclusion: ALA-PDT can promote macrophage polarization to M1 by activating NF-kB pathway.