摘要
采用3株对黄瓜枯萎病菌有较好拮抗作用的木霉,棘孢木霉Trichoderma asperellum 525、哈茨木霉T.harzianum 610和拟康氏木霉T.pseudokoningii 886,研究木霉不同施用方式对黄瓜幼苗叶片膜脂过氧化作用、保护性酶活性及防治枯萎病效果的影响。结果显示,所有木霉处理均能够提高黄瓜幼苗的株高和茎粗,增加幼苗地上部和地下部鲜重,对黄瓜枯萎病的防效均达到64%以上。播种后8~14 d,各处理的黄瓜幼苗叶片质膜透性和丙二醛(MDA)含量不同程度下降,保护性酶活性不同程度的提高,其中以先接种拟康氏木霉886,2 d后接种病原菌的T886-F处理的提高程度最显著。播种后14 d,该处理的叶片质膜透性和MDA含量比只接种病原菌的F处理分别下降了37.02%和14.80%,同时,叶片的SOD、POD、CAT、PPO活性分别比F增加了46.82%、34.93%、18.75%和11.63%。以上结果说明,本研究的3株木霉均能通过改善黄瓜幼苗叶片膜脂过氧化能力,增加保护酶活性,促进幼苗生长,防治黄瓜枯萎病。在实际应用中,提前施入木霉菌剂,有利于促进黄瓜的生长、提高幼苗抗病性、提高病害防治效果。本研究结果为木霉菌剂的高效使用、保障黄瓜安全生产提供指导。
The effect of application modes of Trichoderma on lipid peroxidation,protective enzyme activity of cucumber seedlings leaf and control effect against Fusarium wilt were studied with three antagonistic Trichoderma against Fusarium wilt,T.asperellum 525,T.harzianum 610 and T.pseudokoningii 886.The results showed that all Trichoderma treatments could increase the plant heights and stem diameters of cucumber seedlings,increase the fresh weights of the above ground and underground parts of seedlings,and the control effects of all treatments against cucumber Fusarium wilt exceeded 64%.Membrane permeability and malondialdehyde(MDA)contents of cucumber seedlings decreased at different levels,and protective enzymes in cucumber seedlings continuously increased under all Trichoderma treatments at 8-14 days after sowing,and those of the T886-F treatment(inoculation with Trichoderma 886 first,and then with pathogen 2 days later)changed the most significantly among all the Trichoderma treatments.Compared with F(inoculation with pathogen),the membrane permeability and MDA content of cucumber seedlings decreased 37.02% and 14.80% respectively,and the activities of superoxide dismutase,peroxidase,catalase and polyphenol oxidase of cucumber seedlings increased 46.82%,34.93%,18.75% and 11.63% respectively under the T886-F treatment at 14 days after sowing.The results showed that all the three Trichoderma strains in this study could improve the abilities of membrane lipid peroxidation,increase protective enzyme activities,promote the growth of seedlings and suppress the cucumber Fusarium wilt.In the practical application,the application of Trichoderma agent in advance can promote the growth of cucumber,improve the disease resistance of seedlings and improve the effect of disease control.The results of this study will provide guidance for the efficient use of Trichoderma agents and the safety of cucumber production.
作者
宿畅
廉华
马光恕
李梅
高玉刚
SU Chang;LIAN Hua;MA Guangshu;LI Mei;GAO Yugang(College of Horticulture and Landscape Architecture,Heilongjiang Bayi Agricultural University,Daqing 163319,China;Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China)
出处
《植物保护》
CAS
CSCD
北大核心
2021年第2期142-149,155,共9页
Plant Protection
基金
国家重点研发计划(2018YFD0201202)。
关键词
木霉
黄瓜
枯萎病
保护性酶
丙二醛
质膜透性
Trichoderma
cucumber
Fusarium wilt
protective enzymes
malondialdehyde
membrane permeability