摘要
目的采用电感耦合等离子体质谱法(ICP-MS)和原子吸收光谱法(AAS),分别检测临床患者全血样本中6种微量元素(钙、镁、铁、锌、铜、铅),并从方法学、检测结果两个方面进行分析和比较,便于医学实验室能够根据自身实际需求选择合适的检测方法。方法分别建立了ICP-MS和AAS检测全血中6种微量元素的前处理方法及仪器分析方法,并从准确度、精密度、灵敏度、线性范围进行方法学的比较。并征集不同性别、不同年龄段的90名志愿者,分别使用两种方法对90例实际临床样本进行检测,使用专业的数据统计软件SPSS 20.0对测定结果进行处理。结果ICP-MS和AAS两种方法的准确度、精密度均良好,灵敏度和线性范围也均能满足临床检测要求,90例样本的比对结果显示,两种方法的检测结果呈线性相关(R^(2)>0.97),且差异无统计学意义(P>0.05)。结论两种方法均能满足临床样本的检测要求,但二者各有优劣势,医学实验室可以根据自身实际需求选择合适的检测方法。
Objective To detect six trace elements(Ca,Mg,Fe,Zn,Cu,Pb)in whole blood samples of clinical patients by inductively coupled plasma mass spectrometry(ICP-MS)and atomic absorption spectrometry(AAS),and to analyze and compare the results from both methodology and detection results,so that the medical laboratory can choose the appropriate detection method according to its own actual needs.Methods ICP-MS and AAS were established for the determination of six trace elements in whole blood samples.The methods were compared in terms of accuracy,precision,sensitivity and linear range.90 volunteers of different genders and ages were recruited,and 90 actual clinical samples were tested by two methods,and the results were analyzed by statistics software spss20.0.Results The accuracy and precision of ICP-MS and AAS were both optimal,and the sensitivity and linear range could meet the requirements of clinical detection.The comparison results of 90 samples showed that there was a linear correlation between the two methods(R^(2)>0.97),and there was no significant difference(P>0.05).Conclusion Our results indicate that both methods can meet the detection requirements of clinical samples,but they have their own advantages and disadvantages.Medical laboratories can choose the appropriate detection method according to their own actual needs.
作者
贾永娟
雒琴
刘春冉
倪君君
JIA Yongjuan;LUO Qin;LIU Chunran;NI Junjun(Beijing Harmony Health Medical Diagnostics Co., Ltd, Beijing 100079, China)
出处
《标记免疫分析与临床》
CAS
2021年第3期532-536,共5页
Labeled Immunoassays and Clinical Medicine
关键词
微量元素
电感耦合等离子体质谱法
原子吸收光谱法
方法比较
Trace elements
Inductively coupled plasma mass spectrometry
Atomic absorption spectrometry
Method comparison