醒神解郁汤对卒中后抑郁模型大鼠Th1/Th2及BDNF水平的影响

Effects of Xingshen Jieyu Decoction on levels of Th1/Th2 and BDNF in rats with post-stroke depression

目的研究醒神解郁汤对卒中后抑郁(PSD)大鼠血清Th1/Th2及脑源性神经营养因子(BDNF)水平的影响,探讨其可能的作用机制。方法SD大鼠随机分为正常组、卒中组、卒中后抑郁组、舍曲林组、醒神解郁汤低剂量组、醒神解郁汤高剂量组。其中,卒中组建立右侧大脑中动脉缺血再灌注(MCAO)模型;MCAO模型术后恢复3 d后加慢性不可预测的轻度应激(CUMS)法建立卒中后抑郁模型,PSD造模成功后应用舍曲林(5.142 g/kg)或醒神解郁汤高、低剂量(56.148 g/kg、18.716 g/kg)干预。采用酶联免疫吸附试验法(ELISA)检测大鼠血清炎症因子Th1(IL-6、IFN-γ)、Th2(IL-4、IL-10)、BDNF含量。结果与正常组比较,卒中后抑郁组血清Th1/Th2比值明显增加,抗炎因子IL-4、IL-10以及BDNF含量显著下降,促炎因子IL-6、IFN-γ含量显著增加,差异有统计学意义(P<0.05)。与卒中后抑郁组比较,醒神解郁汤低剂量组、高剂量组血清Th1/Th2比值明显下降,IL-4、IL-10、BDNF含量明显增加,IL-6、IFN-γ含量明显降低,差异有统计学意义(P<0.05)。结论醒神解郁汤可通过调节血清炎症因子Th1/Th2及BDNF水平,改善卒中后抑郁大鼠的神经功能及抑郁行为。

Objective To study the effect of Xingshen Jieyu Decoction on serum Th1/Th2 and brain-derived neurotrophic factor(BDNF)levels in post-stroke depressive(PSD)rats,and to analyze the possible mechanism of Xingshen Jieyu Decoction in treating the disease.Methods SD rats were randomly divided into normal group,stroke group,post-stroke depression group,sertraline group,low-dose group of Xingshen Jieyu Decoction,and high-dose group of Xingshen Jieyu Decoction.The stroke model was established by the right middle cerebral artery ischemia-reperfusion operation.The chronic unpredictable mild stress(CUMS)method was added at 3 days after the middle cerebral artery occlusion(MCAO)operation for PSD model.In the medicine groups the sertraline(5.142 g/kg)and high or low dose(56.148 g/kg,18.176 g/kg)of Xingshen Jieyu Decoction were used after the establishment of PSD model respectively.The enzyme-linked immunosorbent assay(ELISA)was used to detect the contents of serum inflammation factors including Th1(IL-6,IFN-γ),Th2(IL-4,IL-10)and BDNF.Results Compared with those in the normal group,the serum Th1/Th2 ratio in the post-stroke depression group increased significantly,the levels of anti-inflammatory factors such as IL-4,IL-10,and BDNF decreased significantly,and the levels of pro-inflammatory factors such as IL-6 and IFN-γincreased significantly(P<0.05);compared with those in the post-stroke depression group,the serum Th1/Th2 ratio was decreased significantly and the contents of IL-4,IL-10 and BDNF were increased significantly,but the contents of IL-6 and IFN-γwere decreased significantly(P<0.05)in the post-stroke depression groups with low-dose,high-dose of Xingshen Jieyu Decoction.Conclusion Xingshen Jieyu Decoction can improve the neurological function and depressive behavior of poststroke depression rats by regulating the levels of Th1/Th2 and BDNF.