烟酰胺单核苷酸对内毒素休克小鼠死亡率的影响

Effect of nicotinamide mononucleotide on mortality of mice with endotoxic shock

目的探究烟酰胺单核苷酸(NMN)对脂多糖(LPS)诱导的内毒素休克小鼠死亡率的影响。方法将10周龄C57BL/6J雄性小鼠随机分组,均腹腔注射LPS(10 mg/kg)造模。NMN腹腔注射给药,分为3种方式:(1)造模后0.5 h给药,剂量为10、30、100、300 mg/kg;(2)造模前0.5 h给药,剂量为30、100、300、600 mg/kg;(3)造模后0.5 h和12 h两次给药,每次300 mg/kg。观察记录每组小鼠的死亡情况,并绘制生存曲线。结果与溶剂对照组相比,造模后0.5 h或造模前0.5 h给予不同剂量NMN,均不能改善小鼠死亡率或延缓死亡时间;造模后0.5 h和12 h两次给予NMN会加速小鼠死亡,增加小鼠死亡率。两个厂家的NMN产品效果类似。结论NMN对LPS诱导的内毒素休克小鼠不具有治疗作用,小鼠内毒素休克发生后进行NMN多次给药会增加死亡率。

Objective To study the effect of nicotinamide mononucleotide(NMN)on the mortality of the lipopolysaccharide(LPS)-induced endotoxic shock mouse model.Methods 10-week-old C57 BL/6 J male mice were randomly divided into groups,and were injected intraperitoneally(i.p.)with LPS(10 mg/kg)to induce endotoxic shock models.NMN was i.p.injected in three ways:(1)0.5 h after modeling,doses of 10,30,100 and 300 mg/kg;(2)0.5 h before modeling,doses of 30,100,300 and600 mg/kg;or(3)0.5 and 12 h after modeling,dose of 300 mg/kg each time.The death times of each group were recorded,and the survival curves were drawn.Results Compared with the solvent control group,NMN at different doses given 0.5 h after or before modeling didn’t improve the survival rate or delay the death time of endotoxic shock mice;But when given at 0.5 and 12 h300 mg/kg after modeling,NMN accelerated the death of mice and increased the mortality of mice.NMN products by two manufacturers showed similar effects.Conclusion NMN has no therapeutic effect on LPS-induced endotoxic shock,and repeated administration of NMN after endotoxic shock will increase the mortality.