外源氯化胆碱对秋葵幼苗盐和干旱胁迫的缓解效应

Alleviating Effect of Exogenous Choline Chloride on Okra Plants Under Stress

秋葵是一种营养丰富的新型保健型蔬菜,研究秋葵的抗逆性具有重要意义。本试验通过发芽和苗期盆栽试验研究了盐、干旱胁迫下,外源氯化胆碱对秋葵幼苗生长和生理代谢的影响。结果显示,外源氯化胆碱能显著提高秋葵幼苗的耐盐性和抗旱性。在盐、干旱胁迫下,外源氯化胆碱显著提高秋葵植株的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)和过氧化物酶(POD)活性,即162.21%、138.32%、73.32%、80.33%,同时提高秋葵植株的发芽率(40%);外源氯化胆碱处理秋葵植株的脯氨酸、相对水、可溶性糖和叶绿素的含显著增加,分别为157.23%、50.02%、125.96、52.30%,丙二醛(MDA)和过氧化氢(H 2O 2)的含量显著降低,分别为119.06%、213.57%。结果表明,外源氯化胆碱通过维持细胞渗透平衡,保护膜完整性,激活ROS清除系统,从而显著提高了秋葵植株的耐盐性和抗旱性。

Okra is a new kind of nutrient-rich health vegetable,and it is of great importance to study its tolerance to abiotic stresses.Culture pot experiment was used to study the effects of exogenous choline chloride on growth and physiological metabolism of okra plants.In this paper,exogenous choline chloride can significantly enhance salt and drought tolerance of okra plants under salt and drought stresses.Under salt and drought stress,exogenous cholinechloride significantly increased the activities of superoxide dismutase(SOD),catalase(CAT),ascorbate peroxidase(APX)and peroxidase(POD)of okra plants,that is 162.21%,138.32%,73.32%,80.33%,and increased the germination rate of okra plants(40%).The contents of proline,water,soluble sugar and chlorophyll were 157.23%,50.02%,125.96%and 52.30%respectively,while the contents of malondialdehyde(MDA)and hydrogen peroxide(H 2O 2)were 119.06%and 213.57%,respectively.These results demonstrated that the exogenous choline chloride enhances salt tolerance and drought tolerance of okra plants by regulating osmotic balance,protecting membrane integrity and activating ROS scavenging system.