新疆地区APOE基因多态性与子痫前期相关性分析

Association between APOE gene polymorphism and preeclampsia in Xinjiang

目的探讨新疆地区载脂蛋白(APOE)基因多态性与子痫前期发病风险的相关性。方法统计APOE等位基因在世界不同地区健康孕龄妇女中的分布频率。本研究共招募387名女性受试者(健康孕妇280例、重度子痫前期患者81例和轻度子痫前期患者26例)。使用荧光PCR方法检测APOE c.388T>C和c.526C>T基因分型,统计等位基因和基因型在子痫前期组和正常组中的分布频率。采用SPSS 25.0软件进行一般临床特征和基因分型结果的统计分析。结果APOEε2、ε3和ε4等位基因的分布具有显著地域差异。APOE基因多态性与重度子痫前期和轻度子痫前期的发生均无显著相关性。在重度子痫前期患者中,相比于ε3,APOEε2等位基因携带者的甘油三酯水平显著升高(P<0.05)。结论新疆地区APOE基因多态性与子痫前期发病无相关性。

Objective To explore the relationship between apolipoprotein(APOE)gene polymorphism and the risk of preeclampsia in Xinjiang.Methods The distribution frequency of APOE allele in healthy women of gestational age in different regions of the world was calculated.A total of 387 female subjects(280 healthy pregnant women,81 patients with severe preeclampsia and 26 patients with mild preeclampsia)were recruited in this study.The fluorescent PCR method has been used to detect the APOE c.388T>C and c.526C>T genotyping.The distribution frequencies of alleles and genotypes in the preeclampsia group and the normal group were counted.Statistical analysis of general clinical characteristics and genotyping results was performed using SPSS 25.0 software.Results The distribution of APOEε2,ε3 andε4 alleles has significant regional difference.There was no significant correlation between APOE gene polymorphism and the occurrence of severe preeclampsia and mild preeclampsia.In severe preeclampsia,the triglyceride levels of APOEε2 allele carriers are significantly higher thanε3 allele carriers(P<0.05).Conclusion There is no association between APOE gene polymorphism and preeclampsia in Xinjiang area.