花生油脂合成相关基因的表达谱分析

Expression pattern analysis of genes related to lipid synthesis in peanut

为研究不同发育时期花生籽仁油脂合成过程中基因表达调控模式,本研究以高油酸、中油花生品系F18和低油酸、低油花生品种‘鲁花6号’为材料,对下针后10、30、40、60 DAP(days after pegging)的花生种子进行表达谱芯片测序。结果表明,130、3556、2783个基因分别在30、40、60 DAP时期差异表达。GO注释和KEGG富集结果显示,差异表达的基因主要富集在脂肪酸合成和光合等代谢进程中,其中FAB2、FAD2、WRI1等主要参与油酸的积累,参与光合作用的基因均为捕光叶绿素a/b结合蛋白,全部上调表达。代谢通路图结果表明,籽仁发育的40 DAP和60 DAP时期,脂肪酸合成途径的基因均上调表达。研究结果为花生油脂代谢的分子机制提供理论基础,同时也为花生品质改良贡献了基因资源。

In order to survey the regulation patterns of genes expression in the synthesis of oil at different developmental stages in peanut seed,F18(high-oleic medium oil peanut variety)and‘Luhua 6’(low-oleic acid low oil variety)were used as research materials.The expression pattern analysis of genes was performed for peanut seeds on the 10,30,40,and 60 DAP,respectively.The results indicated that 130,3556,and 2783 genes were significantly differentially expressed in the two varieties(lines)on the 30,40,and 60 DAP,respectively.GO annotation and KEGG enrichment showed that DEGs were mainly enriched in the fatty acid synthesis and photosynthetic pathways,such as FAB2,FAD2,WRI1 genes,which were involved in the accumulation of oleic acid.All the genes involved in photosynthesis pathway were photochlorophyll binding proteins and all of them were up-regulated.KEGG pathway indicated that all the genes involved in fatty acid biosynthesis pathway on the 40 DAP and 60 DAP were up-regulated.In summary,these results provide a theoretical basis for molecular study of fatty acid synthesis in the development stage of peanut seeds and offer some candidate genes as gene resources of quality improvement in peanut breeding.