循环血浆中miR-150-5p表达与原发性高血压的关系

Correlation between Essential Hypertension and Expression of miR-150-5p in Circulating Plasma

目的分析循环血浆中微RNA(miR)-150-5p表达与原发性高血压的关系。方法选取2019年5—11月在盐城市第一人民医院住院的80例原发性高血压[收缩压≥140 mm Hg(1 mm Hg=0.133 k Pa)和(或)舒张压≥90 mm Hg]患者作为高血压组,选取同期31例非高血压(收缩压<140 mm Hg和舒张压<90 mm Hg)患者作为非高血压组。应用问卷收集两组患者的一般资料,包括性别、年龄、体重、体质指数(BMI)、空腹血糖、血红蛋白、总蛋白、白蛋白、总胆固醇、三酰甘油、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、血尿酸等;采用实时定量聚合酶链反应测定并比较两组患者的血浆miR-150-5p相对表达水平,采用Pearson积矩相关分析miR-150-5p相对表达水平与血压的相关性,并采用生物信息学方法预测miR-150-5p作用的靶基因及其可能参与调节的细胞信号通路。结果高血压组的体重、BMI、收缩压、舒张压均高于非高血压组[(70±11)kg比(59±11)kg,(25±4)kg/m^(2)比(22±3)kg/m^(2),(158.0±16.5)mm Hg比(121.8±11.9)mm Hg,(91.0±2.2)mm Hg比(74.7±8.4)mm Hg,均P<0.01]。高血压组血浆miR-150-5p的相对表达水平低于非高血压组[2.33(1.96,3.29)×10^(4)AU比5.46(3.98,7.01)×10^(4)AU](Z=-6.080,P<0.001);受试者血浆miR-150-5p的相对表达水平与其收缩压、舒张压均呈负相关(r=-0.521、r=-0.503,均P<0.01);miR-150-5p结合靶基因转录因子特异性蛋白1、锌指E盒同源结合蛋白1、基质金属蛋白酶14、蛋白激酶Cα、VPS53、程序性细胞死亡因子4、EPH受体B2、核受体亚家族2 F组成员2、高尔基体SNAP受体复合物成员1、缺氧诱导的脂滴相关蛋白、锌指和含BTB结构域的蛋白7A、脂联素受体2、Cbl原癌基因、肿瘤蛋白p53、E1A结合蛋白EP300、早期生长反应蛋白2和转录因子原癌基因MYB,参与血压调节。结论原发性高血压患者循环血浆中的miR-150-5p表达水平降低,且其表达水平与血压呈负相关,生物信息学分析发现miR-150-5p可与多种靶基因结合,参与高血压的发生发展。

Objective To explore the correlation between circulating plasma microRNA(miR)-150-5 p and essential hypertension.Methods A total of 80 patients with essential hypertension(systolic blood pressure≥140 mmHg and/or diastolic blood pressure≥90 mm Hg)who were hospitalized in Yancheng NO.1 People’s Hospital from May to Nov.2019 were included as a hypertension group,and 31 patients with no hypertension(systolic blood pressure<140 mm Hg and diastolic blood pressure<90 mm Hg)during the same period were included as a non-hypertension group.The general data of the two groups were collected by questionnaire,including gender,age,body weight,body mass index(BMI),fasting glucose,hemoglobin,total protein,albumin,total cholesterol,triglyceride,low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C)and blood uric acid.Real-time quantitative polymerase chain reaction was used to detect and compare the relative expression of miR-150-5 p in the plasma of the two groups.Pearson product-moment correlation analysis was used to analyze the correlation between miR-150-5 p relative expression level and blood pressure,and bioinformatics method was used to predict the target genes of miR-150-5 p and the cell signaling pathways that may participate in the regulation.Results Body weight,BMI,systolic blood pressure and diastolic blood pressure of the hypertension group were significant higher than those of the non-hypertension group[(70±11)kg vs(59±11)kg,(25±4)kg/m^(2)vs(22±3)kg/m^(2),(158.0±16.5)mm Hg vs(121.8±11.9)mm Hg,(91.0±2.2)mm Hg vs(74.7±8.4)mm Hg,all P<0.001].The relative expression of miR-150-5 p of the hypertension group was significantly lower than that of the non-hypertension group[2.33(1.96,3.29)×10^(4) AU vs 5.56(3.98,7.01)×10^(4) AU,Z=-6.080,P<0.001].And both systolic and diastolic blood pressure were negatively related with the relative expression of miR-150-5 p(r=-0.521,r=-0.503,P<0.01).The target genes of miR-150-5 p possibly were specific protein 1,zinc finger E-box binding homeobox 1,matrix metallopeptidase 14,protein kinase Cα,VPS53,programmed cell death 4,EPH receptor B2,nuclear receptor subfamily 2 group F member 2,Golgiosome SNAP receptor complex member 1,hypoxia inducible lipid droplet associated protein,zinc finger and BTB domain containing 7 A,adiponectin receptor 2,Cbl proto-oncogene,tumor protein p53,E1 A binding protein p300,early growth response protein 2 and transcription factor MYB proto-oncogene,which participated in blood pressure regulation.Conclusion Compared with non-hypertensive patients,the relative expression level of miR-150-5 p in circulating plasma of essential hypertensive patients is lower,and the relative expression level is negatively correlated with blood pressure.Bioinformatic analysis discovered that miR-150-5 p can be combined with a variety of target genes to participate in the development of hypertension.