常见金属离子对过氧化氢酶生物合成的影响

Effect of Common Metal Ions on Catalase Biosynthesis

过氧化氢酶是生物体内的一种末端氧化酶,可以减轻甚至清除过氧化氢在体内聚积对机体造成的损伤,调控过氧化氢酶的生物合成对机体具有十分重要的意义。通过在黑曲霉发酵培养过程中分别加入不同的金属离子,培养结束后进行细胞破壁,提取过氧化氢酶,检测不同金属离子对过氧化氢酶活力的影响。结果表明:在发酵培养的24h阶段,增加CaCO_(3)添加量,同时减少Mg^(2+)、Zn^(2+)、Mn^(2+)、K^(+)、Na^(+)、Cl^(-)、PO4^(3-+)等离子的含量,既可提高菌体的生长速度,又可提高过氧化氢酶的产量。

Catalase is a terminal oxidase in organisms,which can reduce or even remove the damage caused by the accumulation of hydrogen peroxide in the body.It is of great significance to regulate the biosynthesis of catalase.This experiment is through in the training process of aspergillus Niger fermentation joined the different metal ions,respec⁃tively,at the end of the training,make the cell wall-breaking,extraction of catalase,detection of different metal ions on the influence of hydrogen peroxide enzyme activity,the results show that in the training for 24 h of fermentation by increasing CaCO_(3) content at the same time reduce the Mg^(2+),Zn^(2+),Mn^(2+),K^(+),Na^(+),Cl^(-)and PO4^(3-),the level of plasma,can increase the speed of the growth of bacteria and increase production of catalase.