紫花地丁全草化学成分研究

Chemical constituents from whole herbs of Viola yedoensis

目的研究紫花地丁Viola yedoensis全草的化学成分。方法采用硅胶、ODS、Sephadex LH-20凝胶和半制备型高效液相等柱色谱方法进行分离纯化,通过理化性质结合NMR、MS等谱学数据分析鉴定化合物结构。结果从紫花地丁95%乙醇提取物中分离得到21个化合物,分别鉴定为秦皮乙素-6-O-β-D-呋喃芹糖基-(1→2)-β-D-吡喃葡萄糖苷(1)、吲哚-3-甲酸乙酯(2)、脱氢地芰普内酯(3)、滨蒿内酯(4)、蒲公英苦素(5)、水杨苷(6)、丁香苷(7)、4-羟基-4-[3′-(β-D-葡萄糖基)亚丁基]-3,5,5-三甲基-2-环己烯-1-醇(8)、柑橘苷A(9)、(7S,8R)-二氢去氢二松柏醇-9-O-β-D-吡喃葡萄糖苷(10)、山柰素-3-O-α-L-(4-O-乙酰基)鼠李糖基-7-O-α-L-鼠李糖苷(11)、山柰酚-7-O-α-L-鼠李糖苷(12)、山柰酚-3-O-α-L-鼠李糖苷(13)、山柰酚-3-O-α-L-鼠李糖基-7-O-α-L-鼠李糖苷(14)、山柰酚-3-O-β-D-葡萄糖苷(15)、芹菜素(16)、(+)-异地芰普内酯(17)、尿苷(18)、腺苷(19)、6-羟基-香豆素-7-O-α-L-鼠李糖基-(1→6)-O-β-D-葡萄糖苷(20)、(7S,8R)-二氢去氢二松柏醇-4-O-β-D-吡喃葡萄糖苷(21)。结论化合物1为新化合物,命名为紫丁葡芹苷,2为新天然产物,5~11和18为首次从堇菜属中分离得到,12和21为首次从紫花地丁中分离得到。

Objective To investigate the chemical constituents from whole herbs of Viola yedoensis. Methods The chemical constituents were isolated and purified by silica gel, ODS column chromatography, Sephadex LH-20 column chromatography and semi-preparative HPLC. The structures of the isolated compounds were identified by physicochemical properties, NMR and MS spectroscopic methods. Results A total of 21 compounds were isolated from the 95% ethanol extract of V. yedoensis, and their structures were identified as aesculetin 6-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside(1), ethyl indole-3-carboxylate(2), dehydrololiolide(3), scoparone(4), taraxacin(5), salicin(6), syringin(7),(E)-4-hydroxy-4-[3′-(β-D-glucopyranosyloxy) butylidene]-3,5,5-trimethyl-2-cyclohexen-l-one(8), citroside A(9),(7 S,8 R)-dihydrodehydrodiconiferyl alcohol-9-O-β-Dglucopyranoside(10), 3-O-α-L-(4-O-acetyl)rhamnopyranosyl kaempferol 7-O-α-L-rhamnopyranoside(11), kaempferol-7-O-α-Lrhamnopyranoside(12), kaempferol-3-O-α-L-rhamnopyranoside(13), kaempferitrin(14), kaempferol-3-O-β-D-glucopyranoside(15), apigenin(16),(+)-isololiolide(17), uridine(18), β-adenosine(19), 6-hydroxycoumarin-7-O-α-L-rhamnosyl-(1→6)-O-β-D-glucoside(20), and(7 S,8 R)-dihydrodehydrodiconiferyl alcohol 4-O-β-D-glucopyranoside(21). Conclusion Compound 1 is obtained as a new compound, named as vioyegluapiside and compound 2 as a new natural product, with compounds 5-11 and 18 isolating from the genus Viola for the first time and with compounds 12 and 21 from plant V. yedoensis for the first time.