一株具有高效抑菌活性乳酸菌的分离鉴定及生长特性研究

Isolation,identification and growth characteristics of a strain of lactic acid bacteria with efficient antimicrobial activity

从内蒙古地区家庭自制的酸菜中分离筛选了一株具有高效抑菌活性的乳酸菌,并对其生长特性进行了测定。经菌落形态观察、生理生化鉴定及16S rDNA序列分析,该菌株被鉴定为植物乳杆菌。该菌株对金黄色葡萄球菌和大肠杆菌均有良好的抑菌效果,抑菌圈直径分别为15.5,14.5 mm。生长曲线测定结果表明,菌株生长约8 h时,OD 600 nm值开始迅速增大,进入对数生长期;8~24 h时,pH值下降速度较快,为产酸的主要时期。综上,从酸菜中分离筛选出的乳酸菌具有高效抑菌作用,在发酵食品的生产和食品防腐保藏等领域具有潜在的应用价值。

A strain of lactic acid bacteria with high antibacterial activity was isolated from home-made sauerkraut in Inner Mongolia,and its growth characteristics were determined.The strain was identified as Lactobacillus plantarum by colony morphology observation,physiological and biochemical identification and 16S rDNA sequence analysis.This strain had good antibacterial effects on Staphylococcus aureus and Escherichia coli with the diameters of inhibition zone of 15.5 and 14.5 mm,respectively.The results of growth curve showed that the OD 600 nm value began to increase rapidly when the strain grew to about 8 h,and entered the logarithmic growth phase;the pH value decreased rapidly from 8 to 24 h,which was the main period of acid production.In conclusion,the lactic acid bacteria isolated from the pickled Chinese cabbage have high antibacterial effect and have potential application value in the production of fermented food and food preservation.