高效液相色谱法同时测定柚子中的4种黄酮苷和柠檬苦素

Simultaneous determination of four flavonoid glycosides and limonin in pomelo by HPLC

为同时测定柚子中芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷和柠檬苦素,研究了高效液相色谱的洗脱条件、测定波长等对测定结果的影响。结果表明,优选的测定条件为色谱柱:Bio-Bond 5μm C_(18)(4.6 mm×250 mm);进样量10μL;黄酮苷与柠檬苦素的检测波长分别为283 nm和220 nm;流动相:乙腈(A)和水(B),梯度洗脱:0 min,22%A;8 min,22%A;10 min,95%A;16 min,95%A;18 min,22%A;20 min,22%A,流速1 mL/min。该测定条件下,上述5种成分分离良好;其标准曲线相关系数均大于0.9997,检出限为0.69~1.72 mg/L,定量限为1.51~3.15 mg/L,重复性相对标准偏差小于2%。加样回收率均大于98%,加样回收率相对标准偏差均小于2%。

In order to simultaneously determine narirutin,naringin,hesperidin,neohesperidin and limonin in pomelo,the effects of elution conditions and measurement wavelength of high-pressure liquid chromatography on the determination results were studied.The results showed that the optimal measurement conditions were as follows.Chromatographic column of Bio-Bond 5μm C_(18)(4.6 mm×250 mm) was used,and the injection volume was 10μL;the detection wavelengths of flavonoid glycosides and limonin were 283 nm and 220 nm,respectively;The phase:included acetonitrile(A)and water(B)with gradient elution of 0 min,22%A,8 min,22%A,10 min,95%A,16 min,95%A,18 min,22%A,and 20 min,22%A,with 1 mL/min flow rate.Under the control o these conditions,the above five components were well separated;the correlation coefficient of the standard curve was greater than 0.9997,and the detection limit was 0.69~1.72 mg/L.The quantification limit was 1.51~3.15 mg/L,and the relative standard deviation of repeatability was less than 2%.The sample recovery rates were all greater than 98%,and the relative standard deviation of the sample recovery rates were all less than 2%.