基于可编程逻辑控制器的超声阵列辅助酶解控制系统设计与试验

Design and experiment of ultrasound-array-assisted enzymatic hydrolysis control system based on PLC

针对目前多数超生辅助酶解研究过程中采用的超声设备频率和模式单一,酶解过程中的化学指标多数不能实时测量的问题,设计了基于可编程逻辑控制器(Programmable logic controller,PLC)的S型超声阵列辅助酶解控制系统。实现了5种频率的单频、双频和三频模式切换,并采用近红外光谱技术结合PLC控制,建立了以血管紧张素转换酶(Angiotensin I-converting enzyme,ACE)抑制率为指标的数学模型,通过实时在线原位监测ACE抑制率化学值判断酶解终点。试验发现ACE抑制率预测值和实测值的相关度高(R 2>0.84),可以实现酶解终点的自动控制。

At present,the frequency and mode of ultrasonic equipment used in most of the studies of ultrasound-assisted enzymatic hydrolysis are single,and the chemical parameters in the process cannot be measured in real time.In this paper,a programmable logic controller(PLC)based control system of S-type ultrasonic-array-assisted enzymatic hydrolysis was designed.The single-,dual-and triple-frequency mode switching of five frequencies was realized,and a mathematical model with ACE(Angiotensin I-converting enzyme)inhibition rate as an index was established by using near-infrared spectroscopy and PLC control.Therefore,the end-point of enzymatic hydrolysis could be determined by real-time on-line in situ monitoring of ACE inhibitory rate.It was found that the predicted value of ACE inhibitory rate was highly correlated with the measured value(R 2>0.84),and the end-point of enzymatic hydrolysis could be controlled automatically.